Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PHIP CRISPR/Cas9 KO Plasmid (h): sc-407159
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHIP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHIP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PHIP Antibody (B-1): sc-398614
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHIP CRISPR/Cas9 KO Plasmid (h) | sc-407159 | 20 µg | $397.00 |
Overview
PHIP (pleckstrin homology domain interacting protein) encodes a nuclear and cytoplasmic scaffold that associates with insulin receptor substrate signaling and chromatin-associated complexes to influence transcriptional programs and cell-state regulation. Through interactions linked to PI3K–AKT signaling and regulation of gene expression, PHIP contributes to control of cell growth, metabolic responses, and cell-cycle progression. Genetic alteration or dysregulated expression of PHIP has been reported in multiple cancers and is studied for its relationships to tumor cell proliferation, invasion, and treatment response phenotypes. In addition, PHIP has been implicated in neurodevelopmental disorders, supporting its relevance to pathways coupling signaling inputs with transcriptional outputs.
PHIP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHIP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHIP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHIP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHIP protein expression.
This CRISPR knockout system enables efficient generation of PHIP-deficient cell models for investigation of PHIP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHIP exon(s) critical for PHIP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHIP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHIP CRISPR/Cas9 KO Plasmid (h) and PHIP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHIP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHIP HDR Plasmid (h) and PHIP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHIP homology arms to support homology-directed repair at defined PHIP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.