
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PECI CRISPR/Cas9 KO Plasmid (h) | sc-406564 | 20 µg | $397.00 |
ECI2 encodes peroxisomal D3,D2-enoyl-CoA isomerase (PECI), an auxiliary enzyme required for peroxisomal β-oxidation of unsaturated fatty acyl-CoAs. PECI catalyzes isomerization steps that enable continued chain shortening and coordination with downstream dehydrogenation and thiolysis reactions, supporting lipid catabolism and cellular energy homeostasis. Through its role in peroxisome-dependent fatty acid metabolism, ECI2 contributes to regulation of lipid signaling, redox balance, and metabolic adaptation under nutrient stress. Dysregulation of peroxisomal β-oxidation and unsaturated fatty acid processing is relevant to metabolic disease mechanisms, lipid storage phenotypes, and broader metabolic remodeling observed in cancer and neurodegeneration research contexts.
PECI CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ECI2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ECI2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ECI2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PECI protein expression.
This CRISPR knockout system enables efficient generation of ECI2-deficient cell models for investigation of PECI signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
CRISPRs +/- HDRs
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.