Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PCMT1 Double Nickase Plasmid (h): sc-418153-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCMT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PCMT1 Double Nickase Plasmid (h) and PCMT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PCMT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PCMT1 Antibody (D-E7): sc-100977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCMT1 Double Nickase Plasmid (h) | sc-418153-NIC | 20 µg | $410.00 | |||
PCMT1 Double Nickase Plasmid (h2) | sc-418153-NIC-2 | 20 µg | $410.00 |
PCMT1 encodes protein-L-isoaspartate (D-aspartate) O-methyltransferase 1, a SAM-dependent repair enzyme that methylates abnormal isoaspartyl residues arising from spontaneous deamidation or isomerization of asparagine and aspartate. This protein quality control activity supports proteostasis by facilitating conversion of damaged residues back toward normal aspartate and limiting age-associated accumulation of dysfunctional proteins. PCMT1 function intersects with cellular stress responses, protein turnover, and maintenance of enzyme and cytoskeletal protein integrity under oxidative and metabolic stress. Altered PCMT1 activity has been implicated in neuronal vulnerability and protein damage–related phenotypes, making it relevant for studies of neurobiology, aging, and stress-adaptation mechanisms.
PCMT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PCMT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PCMT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PCMT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PCMT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.