p-beta-catenin Antibody (1B11): sc-57533

p-β-catenin Antibody (1B11) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphorylated β-catenin specifically at Tyr 654 in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP). anti-p-β-catenin antibody (1B11) is available in a non-conjugated form, allowing for versatile applications in various experimental setups. Phosphorylated β-catenin plays a crucial role in regulating cellular adhesion and signaling pathways, particularly in Wnt signaling, which is vital for cell proliferation and differentiation. Phosphorylated β-catenin is primarily located in cytoplasm and nucleus, where this protein influences gene expression and cellular behavior. Interaction with E-cadherin is essential for maintaining epithelial integrity, and any dysregulation can lead to increased cellular motility and invasiveness, contributing to cancer progression. Notably, phosphorylation of β-catenin at specific residues, such as Tyr 654, can modulate stability and localization, impacting function in tumorigenesis. Additionally, β-catenin′s interaction with tumor suppressor protein APC is critical for degradation; however, when this interaction is disrupted, as seen in certain cancers, β-catenin accumulates and translocates to nucleus, promoting oncogenic transcription. Understanding dynamics of phosphorylated β-catenin and regulatory mechanisms is essential for elucidating role in cancer biology and developing targeted therapies.

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p-beta-catenin Antibody (1B11) References:

1. The F-box protein beta-TrCP associates with phosphorylated beta-catenin and regulates its activity in the cell.  |  Hart, M., et al. 1999. Curr Biol. 9: 207-10. PMID: 10074433
2. Reduction of beta-catenin/T-cell transcription factor signaling by aspirin and indomethacin is caused by an increased stabilization of phosphorylated beta-catenin.  |  Dihlmann, S., et al. 2003. Mol Cancer Ther. 2: 509-16. PMID: 12813129
3. Activation of c-Met in colorectal carcinoma cells leads to constitutive association of tyrosine-phosphorylated beta-catenin.  |  Herynk, MH., et al. 2003. Clin Exp Metastasis. 20: 291-300. PMID: 12856716
4. Clinical significance of nuclear non-phosphorylated beta-catenin in acute myeloid leukaemia and myelodysplastic syndrome.  |  Xu, J., et al. 2008. Br J Haematol. 140: 394-401. PMID: 18217891
5. APC is essential for targeting phosphorylated beta-catenin to the SCFbeta-TrCP ubiquitin ligase.  |  Su, Y., et al. 2008. Mol Cell. 32: 652-61. PMID: 19061640
6. Accumulation of phosphorylated beta-catenin enhances ROS-induced cell death in presenilin-deficient cells.  |  Boo, JH., et al. 2009. PLoS One. 4: e4172. PMID: 19137062
7. Phosphorylated β-catenin localizes to centrosomes of neuronal progenitors and is required for cell polarity and neurogenesis in developing midbrain.  |  Chilov, D., et al. 2011. Dev Biol. 357: 259-68. PMID: 21736876
8. Lhcgr expression in granulosa cells: roles for PKA-phosphorylated β-catenin, TCF3, and FOXO1.  |  Law, NC., et al. 2013. Mol Endocrinol. 27: 1295-310. PMID: 23754802
9. Wnt signalling induces accumulation of phosphorylated β-catenin in two distinct cytosolic complexes.  |  Gerlach, JP., et al. 2014. Open Biol. 4: 140120. PMID: 25392450
10. Shikonin Inhibits the Migration and Invasion of Human Glioblastoma Cells by Targeting Phosphorylated β-Catenin and Phosphorylated PI3K/Akt: A Potential Mechanism for the Anti-Glioma Efficacy of a Traditional Chinese Herbal Medicine.  |  Zhang, FY., et al. 2015. Int J Mol Sci. 16: 23823-48. PMID: 26473829

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-beta-catenin Antibody (1B11) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-p-beta-catenin Antibody (1B11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-beta-catenin Antibody (1B11) Specifications

Alternate Product Name: β-catenin

Product Clone Name: 1B11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 654

Epitope Target: β-catenin

Epitope Species: human

Product Reactivity/Detection: Tyr 654 phosphorylated β-catenin

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for p-beta-catenin Antibody (1B11): N/A

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.