NUMB Antibody (48): sc-136554

NUMB Antibody (48) is a mouse monoclonal IgG1 kappa light chain antibody that detects NUMB protein of mouse, rat, human, and canine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-NUMB antibody (48) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). NUMB protein plays a crucial role in neuronal cell fate decisions, acting as a signaling adapter with two distinct protein-protein interaction domains: a phosphotyrosine-binding domain and a proline-rich SH3-binding region. This structural configuration is essential for NUMB′s function in mediating interactions with other proteins, such as EPS15, LNX1, and NOTCH1, which are vital for the regulation of cell fate during development. In mammals, NUMB homologs are implicated in determining cell fates, and studies have shown that conditional mouse mutants lacking NUMB in developing sensory ganglia exhibit reduced axonal arborization in afferent fibers, highlighting NUMB′s importance in neural development. Additionally, alterations in cellular calcium homeostasis can influence NUMB-dependent cell fate decisions, further underscoring NUMB′s significance in the nervous system′s development. In chicken, NUMB (c-NUMB) is localized to the basal cortex of mitotic neuroepithelial cells, indicating NUMB′s role in cellular organization during neurogenesis.

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NUMB Antibody (48) References:

1. NUMB localizes in the basal cortex of mitotic avian neuroepithelial cells and modulates neuronal differentiation by binding to NOTCH-1.  |  Wakamatsu, Y., et al. 1999. Neuron. 23: 71-81. PMID: 10402194
2. Distinct human NUMB isoforms regulate differentiation vs. proliferation in the neuronal lineage.  |  Verdi, JM., et al. 1999. Proc Natl Acad Sci U S A. 96: 10472-6. PMID: 10468633
3. Numb modifies neuronal vulnerability to amyloid beta-peptide in an isoform-specific manner by a mechanism involving altered calcium homeostasis: implications for neuronal death in Alzheimer's disease.  |  Chan, SL., et al. 2002. Neuromolecular Med. 1: 55-67. PMID: 12025816
4. Controlling neuron number: does Numb do the math?  |  Castañeda-Castellanos, DR. and Kriegstein, AR. 2004. Nat Neurosci. 7: 793-4. PMID: 15280888
5. Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization.  |  Huang, EJ., et al. 2005. Genes Dev. 19: 138-51. PMID: 15598981
6. Comprehensive identification of phosphorylation sites on the Numb endocytic adaptor protein.  |  Krieger, JR., et al. 2015. Proteomics. 15: 434-46. PMID: 25403733
7. MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway.  |  Zheng, L., et al. 2021. Mol Med Rep. 23: PMID: 33846809
8. Prognostic Biomarker NUMB Is Inhibited by Breast Cancer Cell Exosomes to Promote Breast Cancer Progression.  |  Qin, X. and Cao, Y. 2022. J Immunol Res. 2022: 6032076. PMID: 35478938
9. SPTAN1/NUMB axis senses cell density to restrain cell growth and oncogenesis through Hippo signaling.  |  Su, D., et al. 2023. J Clin Invest. 133: PMID: 37843276
10. Asymmetric localization of numb autonomously determines sibling neuron identity in the Drosophila CNS.  |  Spana, EP., et al. 1995. Development. 121: 3489-94. PMID: 8582263
11. Numb antagonizes Notch signaling to specify sibling neuron cell fates.  |  Spana, EP. and Doe, CQ. 1996. Neuron. 17: 21-6. PMID: 8755475
12. Sanpodo and Notch act in opposition to Numb to distinguish sibling neuron fates in the Drosophila CNS.  |  Skeath, JB. and Doe, CQ. 1998. Development. 125: 1857-65. PMID: 9550718

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NUMB Antibody (48) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-NUMB Antibody (48) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NUMB Antibody (48) Specifications

Alternate Product Name: NUMB

Product Clone Name: 48

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 176-291

Epitope Target: NUMB

Epitope Species: mouse

Product Reactivity/Detection: NUMB

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for NUMB Antibody (48): AC: Agarose, HRP: Horseradish Peroxidase, X: TransCruz Reagent