Date published: 2026-4-29

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NP-40 Alternative (CAS 9016-45-9)

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Alternate Names:
Nonylphenyl Polyethylene Glycol; Nonoxynol; Lissapol; Synperonic
Application:
NP-40 Alternative is a non-ionic surfactant useful in isolating and purifying membrane proteins
CAS Number:
9016-45-9
Molecular Formula:
C15H24O(C2H4O)n
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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NP-40 Alternative is a nonionic surfactant used in biochemistry and molecular biology for its ability to solubilize membrane proteins and to break down protein complexes. This compound is often employed as a milder substitute for the traditional Nonidet P-40. NP-40 Alternative is particularly useful in the preparation of cell lysates, where it helps to preserve the integrity of protein interactions, and in the isolation of nuclei and membrane-bound organelles. It is also a common component in buffer solutions for immunoprecipitation and for the lysis of cells in various protein purification protocols. In addition, NP-40 Alternative is used in assays that require the permeabilization of cell membranes to allow for the entry of reagents or to release cytosolic contents for analysis.


NP-40 Alternative (CAS 9016-45-9) References

  1. Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae detection in urine, endocervical, and vaginal specimens by a multiplexed isothermal thermophilic helicase-dependent amplification (tHDA) assay.  |  O'Neil, D., et al. 2011. J Clin Microbiol. 49: 4121-5. PMID: 21956990
  2. Examining histone posttranslational modification patterns by high-resolution mass spectrometry.  |  Lin, S. and Garcia, BA. 2012. Methods Enzymol. 512: 3-28. PMID: 22910200
  3. Triggering necroptosis in cisplatin and IAP antagonist-resistant ovarian carcinoma.  |  McCabe, KE., et al. 2014. Cell Death Dis. 5: e1496. PMID: 25356865
  4. Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis.  |  Giménez, E., et al. 2015. Anal Chim Acta. 866: 59-68. PMID: 25732693
  5. Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis.  |  Sidoli, S., et al. 2016. J Vis Exp.. PMID: 27286567
  6. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy.  |  Faisal, A., et al. 2017. Br J Cancer. 116: 1166-1176. PMID: 28334731
  7. Evidence for a tubulin-containing lipid-protein structural complex in ciliary membranes.  |  Stephens, RE. 1985. J Cell Biol. 100: 1082-90. PMID: 3980579
  8. Some properties of solubilized GABA receptor.  |  Ito, Y. and Kuriyama, K. 1982. Brain Res. 236: 351-64. PMID: 6279240
  9. Detergent extraction of membrane proteins related to the action of antidiuretic hormone.  |  Ripoche, P., et al. 1982. Biochim Biophys Acta. 693: 497-502. PMID: 6984343
  10. Standardization of an enzyme-linked immunosorbent assay for the determination of protein tyrosine kinase activity.  |  Schraag, B., et al. 1993. Anal Biochem. 211: 233-9. PMID: 7686354

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NP-40 Alternative, 100 ml

sc-281108
100 ml
$101.00

NP-40 Alternative, 500 ml

sc-281108A
500 ml
$142.00