NFXL1 CRISPR/Cas9 KO Plasmid (h): sc-407389
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFXL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NFXL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFXL1 CRISPR/Cas9 KO Plasmid (h) | sc-407389 | 20 µg | $397.00 |
Overview
NFXL1 encodes a zinc finger–containing transcriptional regulator implicated in controlling gene expression programs linked to cellular differentiation and stress-responsive signaling. As a putative DNA-binding factor, NFXL1 is expected to influence transcriptional networks that shape epithelial and immune-related processes, including pathways governing inflammatory tone and barrier function. Human genetic and functional studies have associated NFXL1 with neurodevelopmental phenotypes, supporting its relevance for investigating gene regulation in the context of brain development and cognitive function. Dissecting NFXL1-dependent transcriptional outputs can help clarify downstream targets and pathway crosstalk that contribute to complex disease-associated expression states.
NFXL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NFXL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NFXL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NFXL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NFXL1 protein expression.
This CRISPR knockout system enables efficient generation of NFXL1-deficient cell models for investigation of NFXL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NFXL1 exon(s) critical for NFXL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NFXL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NFXL1 CRISPR/Cas9 KO Plasmid (h) and NFXL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NFXL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NFXL1 HDR Plasmid (h) and NFXL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NFXL1 homology arms to support homology-directed repair at defined NFXL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.