Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myosin X CRISPR/Cas9 KO Plasmid (h): sc-402204
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin X CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myosin X genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myosin X Antibody (C-1): sc-166720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin X CRISPR/Cas9 KO Plasmid (h) | sc-402204 | 20 µg | $397.00 |
Overview
Human MYO10 encodes myosin X, an unconventional actin-based motor that localizes to filopodial tips and regulates filopodia initiation, extension, and cargo transport. Myosin X links actin remodeling to integrin trafficking and cell–matrix adhesion dynamics, supporting directed migration, neurite outgrowth, and morphogenetic processes. Through coordination with cytoskeletal regulators and membrane trafficking pathways, MYO10 influences processes such as endocytosis, protrusion formation, and guidance cue responsiveness. Dysregulated MYO10 expression or function has been associated with altered cell motility and invasive behavior in disease-relevant models, making it useful for studying mechanisms that couple cytoskeletal organization to signaling.
Myosin X CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYO10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYO10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYO10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myosin X protein expression.
This CRISPR knockout system enables efficient generation of MYO10-deficient cell models for investigation of Myosin X signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYO10 exon(s) critical for Myosin X function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYO10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myosin X CRISPR/Cas9 KO Plasmid (h) and Myosin X CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYO10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myosin X HDR Plasmid (h) and Myosin X HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYO10 homology arms to support homology-directed repair at defined MYO10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.