Muskelin Double Nickase Plasmid (h): sc-409182-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Muskelin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Muskelin Double Nickase Plasmid (h) and Muskelin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MKLN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Muskelin Antibody (C-12): sc-398956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Muskelin Double Nickase Plasmid (h) | sc-409182-NIC | 20 µg | $410.00 | |||
Muskelin Double Nickase Plasmid (h2) | sc-409182-NIC-2 | 20 µg | $410.00 |
MKLN1 encodes muskelin, a multidomain scaffold protein that links cell surface receptors to intracellular trafficking and cytoskeletal organization. Muskelin has been implicated in endocytic and vesicular transport processes and contributes to spatial control of protein complexes that influence cell adhesion, morphology, and signaling dynamics. Through these roles, MKLN1 can modulate pathways that depend on receptor turnover and microtubule- or actin-associated transport, shaping context-dependent cellular responses. Dysregulated muskelin-associated trafficking and scaffolding functions have been connected in the literature to altered cell behavior relevant to cancer biology and neurobiological processes, supporting its investigation as a mechanistic node in disease-relevant models.
Muskelin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MKLN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MKLN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MKLN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MKLN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.