Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MLN64 CRISPR/Cas9 KO Plasmid (h): sc-405166
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MLN64 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MLN64 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MLN64 Antibody (H-1): sc-166215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLN64 CRISPR/Cas9 KO Plasmid (h) | sc-405166 | 20 µg | $397.00 |
Overview
STARD3 encodes MLN64, a late endosomal/lysosomal membrane protein containing a START domain that mediates intracellular cholesterol transport. MLN64 contributes to sterol trafficking from endosomes toward sites of steroidogenesis and membrane biogenesis, linking endocytic lipid handling to mitochondrial cholesterol utilization and broader lipid homeostasis networks. Through effects on cholesterol distribution and organelle contact dynamics, STARD3 activity intersects with pathways controlling membrane composition, signaling compartmentalization, and cellular stress responses. Dysregulated STARD3 expression has been associated with altered lipid metabolism and has been studied in the context of proliferative and metabolic phenotypes relevant to cancer biology and neurodegeneration.
MLN64 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STARD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STARD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STARD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MLN64 protein expression.
This CRISPR knockout system enables efficient generation of STARD3-deficient cell models for investigation of MLN64 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STARD3 exon(s) critical for MLN64 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STARD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MLN64 CRISPR/Cas9 KO Plasmid (h) and MLN64 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STARD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MLN64 HDR Plasmid (h) and MLN64 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STARD3 homology arms to support homology-directed repair at defined STARD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.