MLH3 Antibody (H-2): sc-25313

MLH3 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLH3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLH3 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLH3 protein plays a critical role in the DNA mismatch repair (MMR) pathway, which is essential for maintaining genomic stability during DNA replication. MLH3 is primarily located in the nucleus, where MLH3 partners with MLH1 to form a heterodimer that is crucial for recognizing and repairing DNA mismatches. MLH3′s nuclear localization enables direct interaction with other key proteins involved in MMR, such as MSH2 and MSH3, which are responsible for detecting mismatches. MLH3′s effective functioning in the nucleus is vital, as defects in MLH3 can lead to microsatellite instability and are associated with hereditary nonpolyposis colorectal cancer (HNPCC) and other malignancies. Using anti-MLH3 antibody (H-2) in research can provide valuable insights into DNA repair mechanisms and MMR deficiencies in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MLH3 Antibody (H-2) References:

1. Eukaryotic DNA mismatch repair.  |  Kolodner, RD. and Marsischky, GT. 1999. Curr Opin Genet Dev. 9: 89-96. PMID: 10072354
2. Different mutator phenotypes in Mlh1- versus Pms2-deficient mice.  |  Yao, X., et al. 1999. Proc Natl Acad Sci U S A. 96: 6850-5. PMID: 10359802
3. MLH3: a DNA mismatch repair gene associated with mammalian microsatellite instability.  |  Lipkin, SM., et al. 2000. Nat Genet. 24: 27-35. PMID: 10615123
4. Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast.  |  Harfe, BD., et al. 2000. Curr Biol. 10: 145-8. PMID: 10679328
5. Mismatch repair and cancer.  |  Palombo, F., et al. 1994. Nature. 367: 417. PMID: 8107798
6. Mutation of a mutL homolog in hereditary colon cancer.  |  Papadopoulos, N., et al. 1994. Science. 263: 1625-9. PMID: 8128251
7. Genomic organization and expression of the human MSH3 gene.  |  Watanabe, A., et al. 1996. Genomics. 31: 311-8. PMID: 8838312
8. Tumour susceptibility and spontaneous mutation in mice deficient in Mlh1, Pms1 and Pms2 DNA mismatch repair.  |  Prolla, TA., et al. 1998. Nat Genet. 18: 276-9. PMID: 9500552

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLH3 Antibody (H-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-MLH3 Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLH3 Antibody (H-2) Specifications

Alternate Product Name: MLH3

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1228-1453

Epitope Target: MLH3

Epitope Species: human

Product Reactivity/Detection: MLH3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for MLH3 Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin