Date published: 2026-5-22

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Mcm4 Antibody (D-7): sc-166037

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Datasheets
  • Mcm4 Antibody (D-7) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of Mcm4 of Saccharomyces cerevisiae origin
  • recommended for detection of Mcm4 of y origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Mcm4 Antibody (D-7) for WB applications. These reagents are now offered in bundles with Mcm4 Antibody (D-7) (see ordering information below).
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Mcm4 Antibody (D-7) is a mouse monoclonal IgG2a antibody that detects Mcm4 in various species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mcm4 is a crucial member of the mini-chromosome maintenance (MCM) protein family, which plays a vital role in DNA replication regulation, ensuring DNA replicates once during each cell cycle. Mcm4, along with Mcm2, Mcm3, Mcm5, Mcm6, and Mcm7, forms a complex exhibiting DNA helicase activity, essential for unwinding double-stranded DNA during replication. This helicase activity drives cell cycle progression, particularly during G1 to S phase transition where DNA synthesis occurs. E2F transcription factors and various protein kinases tightly control Mcm4 regulation, helping maintain cellular postreplicative state. Mcm4 interacts with other MCM proteins to form a functional complex essential for DNA replication initiation, making anti-Mcm4 antibody (D-7) an invaluable tool for studying cell proliferation and cancer research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Mcm4 Antibody (D-7)

sc-166037
200 µg/ml
$322.00

Mcm4 Antibody (D-7): m-IgG Fc BP-HRP Bundle

sc-537431
200 µg Ab; 10 µg BP
$361.00

Mcm4 Antibody (D-7): m-IgGκ BP-HRP Bundle

sc-534695
200 µg Ab; 40 µg BP
$361.00

Mcm4 Antibody (D-7): m-IgG2a BP-HRP Bundle

sc-548014
200 µg Ab; 10 µg BP
$361.00

How much sample do you recommend loading on the gel for Western blot detection using Mcm4 (D-7): sc-166037?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_5, d_20, h_12CST
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Rated 5 out of 5 by from Produced positive Western blot data of yeastProduced positive Western blot data of yeast recombinant Mcm4 fusion protein. -SCBT QC
Date published: 2013-07-14
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Mcm4 Antibody (D-7) is rated 5.0 out of 5 by 1.
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