LEO1 CRISPR/Cas9 KO Plasmid (h): sc-407864
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LEO1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LEO1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LEO1 CRISPR/Cas9 KO Plasmid (h) | sc-407864 | 20 µg | $397.00 |
Overview
LEO1 encodes a core component of the human PAF1 complex, which associates with RNA polymerase II to coordinate transcription elongation, promoter-proximal pausing release, and co-transcriptional RNA processing. Through interactions with elongation and chromatin-associated factors, LEO1 contributes to histone modification dynamics and regulation of gene expression programs controlling proliferation and differentiation. Altered PAF1 complex activity, including perturbation of LEO1, has been linked to transcriptional dysregulation and genome instability observed in multiple cancer contexts. As a result, LEO1 is frequently studied in pathways governing cell-cycle control, DNA damage responses, and epigenetic regulation of transcription.
LEO1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LEO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LEO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LEO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LEO1 protein expression.
This CRISPR knockout system enables efficient generation of LEO1-deficient cell models for investigation of LEO1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LEO1 exon(s) critical for LEO1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LEO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LEO1 CRISPR/Cas9 KO Plasmid (h) and LEO1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LEO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LEO1 HDR Plasmid (h) and LEO1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LEO1 homology arms to support homology-directed repair at defined LEO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.