Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Lamin B2 CRISPR/Cas9 KO Plasmid (h): sc-401873
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lamin B2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Lamin B2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lamin B2 Antibody (F-8): sc-377379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin B2 CRISPR/Cas9 KO Plasmid (h) | sc-401873 | 20 µg | $397.00 |
Overview
LMNB2 encodes lamin B2, an essential type V intermediate filament protein of the nuclear lamina that supports nuclear envelope architecture and chromatin organization. Lamin B2 contributes to DNA replication, transcriptional regulation, and mitotic nuclear reassembly, integrating structural and regulatory roles in nuclear mechanics and genome stability. As part of the broader lamin network, it influences processes such as nuclear pore positioning and lamina-associated domain (LAD) tethering that shape epigenetic state and cellular differentiation. Dysregulation of nuclear lamina components, including LMNB2, is linked to altered nuclear morphology and has been associated with neurodevelopmental and proliferative phenotypes relevant to mechanobiology and genome maintenance research.
Lamin B2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LMNB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LMNB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LMNB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Lamin B2 protein expression.
This CRISPR knockout system enables efficient generation of LMNB2-deficient cell models for investigation of Lamin B2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LMNB2 exon(s) critical for Lamin B2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LMNB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Lamin B2 CRISPR/Cas9 KO Plasmid (h) and Lamin B2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LMNB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Lamin B2 HDR Plasmid (h) and Lamin B2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LMNB2 homology arms to support homology-directed repair at defined LMNB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.