Ku86 Antibody (Ku15): sc-33653
- Ku86 Antibody (Ku15) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against a 431 human epidermoid carcinoma cell line
- recommended for detection of Ku86 of human origin by WB, IP, IF and IHC(P)
- See Ku86 (B-1): sc-5280 for Ku86 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ku86 Antibody (Ku15) for WB and IHC(P) applications. These reagents are now offered in bundles with Ku86 Antibody (Ku15) (see ordering information below).
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Ku86 Antibody (Ku15) is a mouse monoclonal IgG1 antibody that detects Ku86 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Ku86, also known as Ku80 or p80, is a critical component of the Ku protein complex, which plays a vital role in DNA repair and maintenance of genomic stability. Ku86 is primarily localized in the nucleus, where anti-Ku86 antibody (Ku15) detects heterodimer formation with Ku70 (p70) to bind to DNA double-strand breaks, facilitating the non-homologous end joining (NHEJ) repair pathway. Ku86′s nuclear localization ensures rapid response to DNA damage, which is crucial for preventing mutations and maintaining cellular integrity. Additionally, Ku86 monoclonal antibody (Ku15) can detect interactions with RNA polymerase II and other transcription factors involved in modulating gene expression. Anti-Ku86 antibody (Ku15) serves as an essential tool for researchers studying DNA repair mechanisms, transcriptional regulation, and the pathophysiology of diseases associated with genomic instability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ku86 Antibody (Ku15) References:
- Ku86 is essential in human somatic cells. | Li, G., et al. 2002. Proc Natl Acad Sci U S A. 99: 832-7. PMID: 11792868
- Translocation of Ku86/Ku70 to the multiple myeloma cell membrane: functional implications. | Tai, YT., et al. 2002. Exp Hematol. 30: 212-20. PMID: 11882358
- Ku86 preserves chromatin integrity in cells adapted to high NaCl. | Dmitrieva, NI., et al. 2005. Proc Natl Acad Sci U S A. 102: 10730-5. PMID: 16027367
- Ku86 modulates DNA topoisomerase I-mediated radiosensitization, but not cytotoxicity, in mammalian cells. | Shih, SJ., et al. 2005. Cancer Res. 65: 9194-9. PMID: 16230379
- Activities of DNA-PK and Ku86, but not Ku70, may predict sensitivity to cisplatin in human gliomas. | Shao, CJ., et al. 2008. J Neurooncol. 89: 27-35. PMID: 18415044
- Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells. | Gullo, CA., et al. 2008. Cancer Cell Int. 8: 4. PMID: 18442416
- Ku86 is important for TrkA overexpression-induced breast cancer cell invasion. | Lagadec, C., et al. 2010. Proteomics Clin Appl. 4: 580-90. PMID: 21137076
- Expression of Ku86 and presence of Ku86 antibody as biomarkers of hepatitis B virus related hepatocellular carcinoma. | Xu, Y., et al. 2014. Dig Dis Sci. 59: 614-22. PMID: 24271118
- Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination. | Zan, H., et al. 2017. Nat Commun. 8: 14244. PMID: 28176781
- Ku86 alleviates human umbilical vein endothelial cellular apoptosis and senescence induced by a low dose of ionizing radiation. | Wu, K., et al. 2019. J Int Med Res. 47: 893-904. PMID: 30616437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ku86 Antibody (Ku15) | sc-33653 | 200 µg/ml | $322.00 | |||
Ku86 Antibody (Ku15): m-IgG Fc BP-HRP Bundle | sc-536769 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ku86 Antibody (Ku15): m-IgGκ BP-HRP Bundle | sc-533933 | 200 µg Ab; 40 µg BP | $361.00 | |||
Ku86 Antibody (Ku15): m-IgG1 BP-HRP Bundle | sc-544810 | 200 µg Ab; 20 µg BP | $361.00 |