Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ki67 Double Nickase Plasmid (h): sc-400081-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ki67 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ki67 Double Nickase Plasmid (h) and Ki67 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MKI67. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ki67 Antibody (Ki-67): sc-23900
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ki67 Double Nickase Plasmid (h) | sc-400081-NIC | 20 µg | $410.00 | |||
Ki67 Double Nickase Plasmid (h2) | sc-400081-NIC-2 | 20 µg | $410.00 |
MKI67 encodes Ki67, a chromatin-associated nuclear protein widely used as a marker of active cell cycling, with expression peaking during S/G2/M and being absent in quiescent G0 cells. Ki67 contributes to higher-order chromosome organization and supports mitotic chromosome periphery formation, helping maintain proper chromosome segregation and cell division dynamics. Through its tight coupling to proliferation programs, Ki67 intersects with cell cycle regulatory networks and is frequently leveraged to study growth control and replication stress responses. Dysregulated MKI67 expression is commonly associated with hyperproliferative states across diverse tumor types, supporting its value in mechanistic studies of cell cycle control and cancer biology.
Ki67 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MKI67 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MKI67. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MKI67 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MKI67-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.