Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFITM3 Double Nickase Plasmid (h): sc-403281-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFITM3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFITM3 Double Nickase Plasmid (h) and IFITM3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFITM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFITM3 Antibody (F-41): sc-100768
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFITM3 Double Nickase Plasmid (h) | sc-403281-NIC | 20 µg | $410.00 |
IFITM3 (interferon-induced transmembrane protein 3) is an interferon-stimulated, endolysosomal membrane protein that restricts entry of enveloped viruses by limiting membrane fusion and modulating endocytic trafficking. Its expression is regulated by type I/II interferon signaling and contributes to innate immune defense programs that remodel membrane composition and vesicular dynamics during infection. IFITM3 is also linked to inflammatory responses and has been studied for roles in immune cell signaling, stress responses, and tumor biology through effects on membrane organization and receptor trafficking. Genetic and expression differences in IFITM3 have been associated with variability in susceptibility and severity of viral infection phenotypes, supporting its relevance in host–pathogen interaction research.
IFITM3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFITM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFITM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFITM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFITM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.