Molecular structure of Hygromycin B solution, CAS Number: 31282-04-9
Hygromycin B solution (CAS 31282-04-9)
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Alternate Names:
Hygromycin B is also known as Hygrovetine and D-streptamine
Application:
Hygromycin B solution is An antibiotic often used for selection of cells transfected with E. coli hygromycin resistance gene.
CAS Number:
31282-04-9
Purity:
>90%
Molecular Weight:
527.52
Molecular Formula:
C20H37N3O13
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Hygromycin B solution is used in molecular and cellular biology research as a selective agent for the isolation of genetically modified cells containing the hygromycin resistance gene. It operates by inhibiting protein synthesis by affecting the translocation step in bacterial and eukaryotic ribosomes, which makes it a useful tool for the selection and maintenance of stably transfected cells. In plant biology, hygromycin B solution is used to select for transgenic plants that have been engineered to carry the hygromycin resistance gene, facilitating studies in plant genetics and crop improvement. The compound is also valuable in the study of ribosomal function and antibiotic resistance mechanisms. Moreover, hygromycin B solution is used in the research of gene expression regulation and vector development, as it allows for the efficient screening of cells that have integrated foreign DNA into their genomes.
Hygromycin B solution (CAS 31282-04-9) References
- The 3'-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum. | Bilang, R., et al. 1991. Gene. 100: 247-50. PMID: 1647356
- Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. | Hiei, Y. and Komari, T. 2008. Nat Protoc. 3: 824-34. PMID: 18451790
- Aminoglycoside acetyltransferase 3-IV (aacC4) and hygromycin B 4-I phosphotransferase (hphB) in bacteria isolated from human and animal sources. | Salauze, D., et al. 1990. Antimicrob Agents Chemother. 34: 1915-20. PMID: 1963287
- Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens. | Liu, Z. and Friesen, TL. 2012. Methods Mol Biol. 835: 365-75. PMID: 22183664
- Defective H(+)-ATPase of hygromycin B-resistant pma1 mutants fromSaccharomyces cerevisiae. | Perlin, DS., et al. 1989. J Biol Chem. 264: 21857-64. PMID: 2532214
- Hygromycin resistance as a selectable marker in Dictyostelium discoideum. | Egelhoff, TT., et al. 1989. Mol Cell Biol. 9: 1965-8. PMID: 2546056
- Isolation of metabolic genes and demonstration of gene disruption in the phytopathogenic fungus Ustilago maydis. | Kronstad, JW., et al. 1989. Gene. 79: 97-106. PMID: 2673937
- Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing. | Obinata, H., et al. 2018. PLoS One. 13: e0197128. PMID: 29742140
- Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops. | Martins, PK., et al. 2019. Methods Mol Biol. 1864: 49-65. PMID: 30415328
- Membrane potential defect in hygromycin B-resistant pma1 mutants of Saccharomyces cerevisiae. | Perlin, DS., et al. 1988. J Biol Chem. 263: 18118-22. PMID: 3056938
- Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. | Cullen, D., et al. 1987. Gene. 57: 21-6. PMID: 3322945
- Covalent molecularly imprinted electrochemical sensor modulated by borate ester bonds for hygromycin B detection based on the synergistic signal amplification of Cu-MOF and MXene. | Wang, H., et al. 2022. Food Chem. 383: 132382. PMID: 35168044
- Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. | González, A., et al. 1978. Biochim Biophys Acta. 521: 459-69. PMID: 367435
- Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. | Rao, RN., et al. 1983. Antimicrob Agents Chemother. 24: 689-95. PMID: 6318654
- Yeast glycosylation mutants are sensitive to aminoglycosides. | Dean, N. 1995. Proc Natl Acad Sci U S A. 92: 1287-91. PMID: 7877969
Inhibitor of:
Aminopeptidase P1, EG621697, FDXACB1, Fks1, H2-Q6, IF2, LOC100040585, MARS2, MetRS, Ribosomal Protein L18, Ribosomal Protein L23, Ribosomal Protein L30, Ribosomal Protein L39, Ribosomal Protein LP0, Ribosomal Protein S14, Ribosomal Protein S21, Ribosomal Protein S5, Ribosomal Protein S7, Ribosomal Protein S9, Salmonella (Enterobacterales), Salmonella O, Sup35, and TARSL1.Activator of:
ALG9, Ribosomal Protein L15, RRP8, and Tra-1.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hygromycin B solution, 1 g | sc-29067 | 1 g | $104.00 |