HLX1 Double Nickase Plasmid (h): sc-403700-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HLX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HLX1 Double Nickase Plasmid (h) and HLX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HLX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HLX1 Antibody (1B9): sc-293328
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLX1 Double Nickase Plasmid (h) | sc-403700-NIC | 20 µg | $410.00 | |||
HLX1 Double Nickase Plasmid (h2) | sc-403700-NIC-2 | 20 µg | $410.00 |
HLX (HLX1) encodes a homeobox transcription factor that regulates cell fate decisions and differentiation programs during development, with prominent roles in hematopoietic and immune cell lineages. By binding sequence-specific DNA motifs, HLX1 modulates transcriptional networks controlling proliferation, survival, and lineage commitment, integrating with pathways that shape cytokine responses and developmental patterning. Dysregulated HLX expression has been reported in multiple cancer contexts, particularly hematologic malignancies, where altered transcriptional control can contribute to aberrant growth and blocked differentiation. In basic research, HLX1 serves as a node for studying homeobox-dependent gene regulation and transcriptional circuitry in normal and transformed human cells.
HLX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HLX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HLX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HLX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HLX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.