Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h): sc-400388-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h) and Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HDAC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone Deacetylase 4 Antibody (A-4): sc-46672
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h) | sc-400388-NIC | 20 µg | $410.00 | |||
Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h2) | sc-400388-NIC-2 | 20 µg | $410.00 |
Human HDAC4 encodes histone deacetylase 4, a class IIa HDAC that shuttles between nucleus and cytoplasm to modulate chromatin accessibility and transcriptional programs. HDAC4 functions in multiprotein co-repressor complexes and integrates calcium/calmodulin-dependent kinase and 14-3-3 signaling to regulate MEF2-driven gene expression, cell differentiation, and stress-responsive transcription. Through deacetylation-dependent control of histone and non-histone substrates, HDAC4 contributes to pathways governing muscle and neuronal development, synaptic plasticity, and metabolic homeostasis. Dysregulated HDAC4 activity and localization have been linked to aberrant epigenetic states observed across neurodevelopmental, neurodegenerative, and oncogenic contexts, making it a useful node for mechanistic studies of transcriptional repression.
Histone Deacetylase 4 (HDAC4) Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HDAC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HDAC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HDAC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HDAC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.