Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hippocalcin CRISPR/Cas9 KO Plasmid (m): sc-420938
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hippocalcin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hippocalcin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hippocalcin Antibody (G-8): sc-393125
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hippocalcin CRISPR/Cas9 KO Plasmid (m) | sc-420938 | 20 µg | $397.00 |
Overview
Hpca encodes hippocalcin, a neuronal EF-hand calcium-binding protein of the neuronal calcium sensor family that couples Ca2+ transients to changes in synaptic signaling and excitability. Upon Ca2+ binding, hippocalcin undergoes a conformational switch that can modulate membrane association and influence processes such as synaptic plasticity, activity-dependent trafficking, and gene expression programs in neurons. It participates in calcium-dependent signaling networks that intersect with neurotransmitter receptor pathways and downstream kinase/phosphatase cascades shaping long-term potentiation and depression. Dysregulated neuronal Ca2+ homeostasis and altered calcium sensor function are relevant to mechanistic studies of cognitive phenotypes and neurodegenerative or neuropsychiatric disease-associated circuit dysfunction in mouse models.
hippocalcin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hpca gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hpca together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hpca open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hippocalcin protein expression.
This CRISPR knockout system enables efficient generation of Hpca-deficient cell models for investigation of hippocalcin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hpca exon(s) critical for hippocalcin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hpca genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hippocalcin CRISPR/Cas9 KO Plasmid (m) and hippocalcin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hpca locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hippocalcin HDR Plasmid (m) and hippocalcin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hpca homology arms to support homology-directed repair at defined Hpca target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.