Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HEXIM1 Double Nickase Plasmid (h): sc-402333-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HEXIM1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HEXIM1 Double Nickase Plasmid (h) and HEXIM1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HEXIM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HEXIM1 Antibody (D-8): sc-390059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HEXIM1 Double Nickase Plasmid (h) | sc-402333-NIC | 20 µg | $410.00 | |||
HEXIM1 Double Nickase Plasmid (h2) | sc-402333-NIC-2 | 20 µg | $410.00 |
HEXIM1 (hexamethylene bis-acetamide inducible 1) encodes a nuclear regulator of transcriptional elongation that modulates P-TEFb activity through the 7SK snRNP complex, influencing RNA polymerase II pause release and gene expression programs. By binding and inhibiting CDK9/Cyclin T, HEXIM1 contributes to control of cell-cycle progression, differentiation, and stress-responsive transcriptional networks. Altered HEXIM1 expression or regulation has been linked to dysregulated transcriptional control in cancer biology and has also been studied in contexts of hormonal signaling and cardiac remodeling. As a nodal factor in elongation control, HEXIM1 is frequently interrogated to dissect pathway-level effects on transcription, chromatin-associated processes, and downstream phenotypes.
HEXIM1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HEXIM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HEXIM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HEXIM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HEXIM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.