HCN2 CRISPR/Cas9 KO Plasmid (m): sc-420813
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HCN2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HCN2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HCN2 CRISPR/Cas9 KO Plasmid (m) | sc-420813 | 20 µg | $397.00 |
Overview
Hcn2 encodes the hyperpolarization-activated cyclic nucleotide–gated channel 2 (HCN2), an ion channel that carries Ih and contributes to pacemaker activity, membrane excitability, and rhythmic firing in neurons and cardiomyocytes. HCN2 integrates hyperpolarization with cyclic nucleotide signaling, linking cAMP/PKA dynamics to changes in action potential threshold and firing frequency. In the nervous system, HCN2 influences sensory processing and network oscillations through regulation of dendritic integration and synaptic responsiveness. Dysregulated HCN2 function has been associated with altered excitability phenotypes relevant to studies of pain pathways, epilepsy-related mechanisms, and cardiac conduction biology in mouse models.
HCN2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hcn2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hcn2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hcn2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HCN2 protein expression.
This CRISPR knockout system enables efficient generation of Hcn2-deficient cell models for investigation of HCN2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hcn2 exon(s) critical for HCN2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hcn2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HCN2 CRISPR/Cas9 KO Plasmid (m) and HCN2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hcn2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HCN2 HDR Plasmid (m) and HCN2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hcn2 homology arms to support homology-directed repair at defined Hcn2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.