Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTO1 Double Nickase Plasmid (h): sc-404107-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTO1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTO1 Double Nickase Plasmid (h) and GSTO1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTO1 Antibody (21): sc-130317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTO1 Double Nickase Plasmid (h) | sc-404107-NIC | 20 µg | $410.00 | |||
GSTO1 Double Nickase Plasmid (h2) | sc-404107-NIC-2 | 20 µg | $410.00 |
Glutathione S-transferase omega 1 (GSTO1) is a cytosolic enzyme in the omega class of GSTs that uses glutathione to catalyze thioltransferase and deglutathionylation reactions, influencing cellular redox balance and protein S-glutathionylation status. Through these activities, GSTO1 contributes to detoxification and regulation of oxidative stress responses, intersecting with glutathione metabolism and broader redox-controlled signaling pathways. Variation in GSTO1 expression or activity has been associated with altered susceptibility to oxidative damage and inflammatory signaling, processes relevant to neurodegeneration, cancer biology, and metabolic stress contexts. In human cells, GSTO1 function is often studied in relation to reactive electrophile handling, redox-dependent regulation of enzymes, and stress-adaptive transcriptional programs.
GSTO1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.