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group VI iPLA<sub>2</sub> Antibody (E-8) - Western Blotting - Image 373595
group VI iPLA2 Antibody (E-8): sc-166616. Western blot analysis of group VI iPLA2 expression in F9 (A), PC-12 (B) and c4 (C) whole cell lysates and mouse liver tissue extract (D).
group VI iPLA2 Antibody (E-8): sc-166616. Western blot analysis of group VI iPLA2 expression in F9 (A), PC-12 (B) and c4 (C) whole cell lysates and mouse liver tissue extract (D).

group VI iPLA2 Antibody (E-8): sc-166616

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Datasheets
  • group VI iPLA2 Antibody (E-8) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
  • raised against amino acids 1-120 of group VI iPLA2 of human origin
  • recommended for detection of calcium-independent PLA2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for group VI iPLA2 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with group VI iPLA2 Antibody (E-8) (see ordering information below).
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Additional Phospholipase A2 Antibodies including group II sPLA2, group IVD sPLA2, group IVF sPLA2, group V PLA2, group X sPLA2 and group XIIA sPLA2

group VI iPLA2 Antibody (E-8) is a mouse monoclonal IgG2b antibody that detects group VI iPLA2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). group VI iPLA2 plays a crucial role in lipid metabolism by catalyzing the hydrolysis of phospholipids, which is essential for maintaining cellular membrane integrity and signaling pathways. group VI iPLA2 is primarily localized in the cytoplasm and interacts with various membrane components, influencing the dynamics of membrane phospholipid composition. The human gene encoding group VI iPLA2 is located on chromosome 22q13.1, and expression is tightly regulated, reflecting importance in physiological processes such as inflammation and cell signaling. Additionally, the presence of multiple isoforms, including those that lack the catalytic domain, suggests a complex regulatory mechanism that may modulate group VI iPLA2 activity, further underscoring significance in cellular functions. anti-group VI iPLA2 antibody (E-8) is an invaluable tool for researchers studying lipid metabolism and implications in various biological processes and diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

group VI iPLA2 Antibody (E-8) References:

  1. Role of calcium-independent phospholipases (iPLA(2)) in phosphatidylcholine metabolism.  |  Chiu, CH. and Jackowski, S. 2001. Biochem Biophys Res Commun. 287: 600-6. PMID: 11563837
  2. Identification and distribution of endoplasmic reticulum iPLA2.  |  Kinsey, GR., et al. 2005. Biochem Biophys Res Commun. 327: 287-93. PMID: 15629460
  3. Mitochondrial iPLA2 activity modulates the release of cytochrome c from mitochondria and influences the permeability transition.  |  Gadd, ME., et al. 2006. J Biol Chem. 281: 6931-9. PMID: 16407316
  4. Passive calcium leak via translocon is a first step for iPLA2-pathway regulated store operated channels activation.  |  Flourakis, M., et al. 2006. FASEB J. 20: 1215-7. PMID: 16611832
  5. iPLA2, a novel determinant in Ca2+- and phosphorylation-dependent S100A8/A9 regulated NOX2 activity.  |  Schenten, V., et al. 2010. Biochim Biophys Acta. 1803: 840-7. PMID: 20219570
  6. Proinflammatory treatment of astrocytes with lipopolysaccharide results in augmented Ca2+ signaling through increased expression of via phospholipase A2 (iPLA2).  |  Strokin, M., et al. 2011. Am J Physiol Cell Physiol. 300: C542-9. PMID: 21178110
  7. A mouse model of in vivo chemical inhibition of retinal calcium-independent phospholipase A2 (iPLA2).  |  Saab-Aoudé, S., et al. 2013. Biochimie. 95: 903-11. PMID: 23266358
  8. Inhibition of iPLA2 β and of stretch-activated channels by doxorubicin alters dystrophic muscle function.  |  Ismail, HM., et al. 2013. Br J Pharmacol. 169: 1537-50. PMID: 23849042
  9. Impaired corticostriatal LTP and depotentiation following iPLA2 inhibition is restored following acute application of DHA.  |  Mazzocchi-Jones, D. 2015. Brain Res Bull. 111: 69-75. PMID: 25562715
  10. Leptin suppresses non-apoptotic cell death in ischemic rat cardiomyocytes by reduction of iPLA(2) activity.  |  Takatani-Nakase, T. and Takahashi, K. Biochem Biophys Res Commun. 463: 13-7. PMID: 25979360

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

group VI iPLA2 Antibody (E-8)

sc-166616
200 µg/ml
$322.00

group VI iPLA2 Antibody (E-8): m-IgG Fc BP-HRP Bundle

sc-537511
200 µg Ab; 10 µg BP
$361.00

group VI iPLA2 Antibody (E-8): m-IgGκ BP-HRP Bundle

sc-534795
200 µg Ab; 40 µg BP
$361.00

I am using group VI iPLA2 (E-8): sc-166616 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-06-19
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-05-02
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group VI iPLA 2 Antibody (E-8) is rated 4.5 out of 5 by 2.
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