GPD1 Antibody (E-7): sc-376219

GPD1 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects GPD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GPD1 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glycerol-3-phosphate dehydrogenase 1 (GPD1) plays a crucial role in cellular metabolism by catalyzing the conversion of sn-glycerol 3-phosphate and NAD+ into glycerone phosphate and NADH, which is essential for energy production and lipid metabolism. This 349 amino acid cytoplasmic protein is particularly important in cardiac tissue, where GPD1 influences the function of voltage-gated sodium channels that are vital for the depolarization phase of the cardiac action potential, thereby affecting the conduction of electrical signals through the heart. GPD1 exists as a homodimer and is inhibited by zinc ions and sulfate, with potential functional similarities to GPD1L (glycerol-3-phosphate dehydrogenase-1 like), a protein implicated in the trafficking of cardiac sodium currents to the cell surface. Mutations in the GPD1L gene have been associated with sudden infant death syndrome (SIDS), highlighting the importance of GPD1 in both metabolic processes and cardiac health.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GPD1 Antibody (E-7) References:

1. Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae.  |  Lin, H., et al. 2002. Mol Genet Genomics. 267: 313-20. PMID: 12073033
2. Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1).  |  Ou, X., et al. 2006. J Mol Biol. 357: 858-69. PMID: 16460752
3. GRB14, GPD1, and GDF8 as potential network collaborators in weight loss-induced improvements in insulin action in human skeletal muscle.  |  Park, JJ., et al. 2006. Physiol Genomics. 27: 114-21. PMID: 16849634
4. Molecular and functional characterization of novel glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) mutations in sudden infant death syndrome.  |  Van Norstrand, DW., et al. 2007. Circulation. 116: 2253-9. PMID: 17967976
5. Mutation analysis of the glycerol-3 phosphate dehydrogenase-1 like (GPD1L) gene in Japanese patients with Brugada syndrome.  |  Makiyama, T., et al. 2008. Circ J. 72: 1705-6. PMID: 18762705
6. Sequence conservation and structural organization of the glycerol-3-phosphate dehydrogenase promoter in mice and humans.  |  Gwynn, B., et al. 1990. Mol Cell Biol. 10: 5244-56. PMID: 2398890
7. GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway.  |  Albertyn, J., et al. 1994. Mol Cell Biol. 14: 4135-44. PMID: 8196651

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPD1 Antibody (E-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-GPD1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPD1 Antibody (E-7) Specifications

Alternate Product Name: GPD1

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 321-347

Epitope Target: GPD1

Epitope Species: human

Product Reactivity/Detection: GPD1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for GPD1 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin