G alpha s Antikörper (12) Empfohlen für die Detektion von Gα s aus der Spezies mouse, rat und human per WB und IP

Gα s Antikörper (12): sc-135914

Gα s Antikörper (12) wurde bewertet mit 4.7 von 5 von 11.
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Datenblatt

    • Gα s Antikörper (12) ist ein Maus monoklonales IgG1 (kappa light chain) in einer Menge von 200 µg/ml
    • gezogen gegen die Aminosäuresequenz 11-21 von Gα s aus der Spezies human
    • Empfohlen für die Detektion von Gα s aus der Spezies mouse, rat und human per WB und IP
    • erhältlich als Konjugat mit Agarose für IP; und mit HRP für WB, IHC(P) und ELISA
    • Beachten Sie m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), unsere besonders zu empfehlenden rekombinanten Alternativen zu konventionellen sekundären anti-Maus IgG Reagenzien.
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom Gα s (12): sc-135914 zu erhalten.

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Gefragt von: ASM25
Thank you for your question. The antibody was raised against amino acids 11-21 of G s of human origin. The exact binding region of this antibody within the antigen has not been mapped. This antibody has not been tested experimentally for potential cross-reactivity under denaturing conditions in a Western Blot or native conditions in IP against different proteins. You might want to align the antigen sequence against the protein form of interest in order to determine potential reactivity. based on the antigen sequence we do not expect relevant cross-reactivity against G -olf. Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.
Beantwortet von: SCBT Heidelberg Support
Veröffentlichungsdatum: 2018-11-27

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

Gefragt von: Zhong Ding
Thank you for your inquiry. For a limited time, we are offering a free 10 µg sample of this antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Beantwortet von: Technical Support 15
Veröffentlichungsdatum: 2018-02-01

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Gefragt von: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Beantwortet von: Technical Support 9
Veröffentlichungsdatum: 2017-01-12

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?

Gefragt von: TinTin
We recommend Jurkat Whole Cell Lysate (sc-2204) or rat or mouse brain tissue extract (sc-2392 or sc-2253) as positive controls as we have Western blot data showing specific detection of Gα s in these samples.
Beantwortet von: Technical Support 9
Veröffentlichungsdatum: 2017-01-12
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Rated 4 von 5 von aus Strong Antibody Signal for WB We tried a sample antibody on WB, signal was strong, but we got two bands.
Veröffentlichungsdatum: 2017-11-03
Rated 5 von 5 von aus no problem The WB of the antibody was detected in the tissues of rat cerebellum, the signals were clear, specific and unique.
Veröffentlichungsdatum: 2017-10-26
Rated 5 von 5 von aus Worked good This antibody sc-135914 works very well in WB, TBS 5% milk, diluted 1/250.
Veröffentlichungsdatum: 2017-08-25
Rated 5 von 5 von aus works well It works well in western blotting at dilution of 1:500 analysis Human liver tissue .
Veröffentlichungsdatum: 2017-02-25
Rated 3 von 5 von aus Moderate specificity Galpha-S (12/sc-135914): Moderate specificity: detects multiple bands from HEK293 lysate, of which one is the correct MW for Galpha-S. Poor sensitivity - may be optimized.
Veröffentlichungsdatum: 2017-02-09
Rated 5 von 5 von aus Superb western Very nice western blot results; strong signal with low backgrond
Veröffentlichungsdatum: 2017-01-31
Rated 5 von 5 von aus Great for WB A proven reagent for WB detection. It provides great signal and clear specific bands. It is reactive with mouse, rat, and human and has been published with several times.
Veröffentlichungsdatum: 2017-01-30
Rated 5 von 5 von aus Various expression levels of the G s protein Various expression levels of the G s protein is demonstrated by Western Blot using Jurkat, HEK293, TT, K562 and MCF-7 whole cell lysates. -SCBT QC
Veröffentlichungsdatum: 2015-04-12
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