G_{α 12} Antibody (E-12): sc-515445

Gα 12 Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gα12 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Gα12 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα12 plays a crucial role in cellular signaling pathways by acting as a molecular switch that relays signals from activated G protein-coupled receptors to downstream effectors, thereby influencing various physiological processes such as cell proliferation, differentiation, and migration. Notably, Gα12 interacts with specific proteins, including Rho guanine nucleotide exchange factors, which are essential for the activation of the Rho family of small GTPases. This interaction is vital for cytoskeletal reorganization and the regulation of cell shape and motility, making Gα12 a significant player in processes such as wound healing and cancer metastasis. With broad tissue distribution and involvement in critical signaling pathways, Gα12 monoclonal antibody (E-12) serves as an invaluable tool for researchers studying G protein signaling and its implications in health and disease.

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G_{α 12} Antibody (E-12) References:

1. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
2. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
3. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
4. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
5. G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits.  |  Strathmann, MP. and Simon, MI. 1991. Proc Natl Acad Sci U S A. 88: 5582-6. PMID: 1905812
6. Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse.  |  Strathmann, M., et al. 1989. Proc Natl Acad Sci U S A. 86: 7407-9. PMID: 2508088
7. Gα12 overexpression induced by miR-16 dysregulation contributes to liver fibrosis by promoting autophagy in hepatic stellate cells.  |  Kim, KM., et al. 2018. J Hepatol. 68: 493-504. PMID: 29080810
8. Heterotrimeric Gα12/13 proteins in kidney injury and disease.  |  Tutunea-Fatan, E., et al. 2020. Am J Physiol Renal Physiol. 318: F660-F672. PMID: 31984793
9. Gα12/13 signaling in metabolic diseases.  |  Yang, YM., et al. 2020. Exp Mol Med. 52: 896-910. PMID: 32576930
10. Gα12 overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated miR-15a and ALOX12 dysregulation.  |  Tak, J., et al. 2022. Theranostics. 12: 1570-1588. PMID: 35198058
11. Gα12 signaling regulates transcriptional and phenotypic responses that promote glioblastoma tumor invasion.  |  Chaim, OM., et al. 2023. Sci Rep. 13: 22412. PMID: 38104152
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α 12} Antibody (E-12) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α 12} Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α 12} Antibody (E-12) Specifications

Alternate Product Name: G-protein subunit GNA12

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 89-300

Epitope Target: G_{α 12}

Epitope Species: human

Product Reactivity/Detection: G_{α 12}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for G_{α 12} Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin