Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
galectin-8 Double Nickase Plasmid (h): sc-401785-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- galectin-8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- galectin-8 Double Nickase Plasmid (h) and galectin-8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LGALS8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: galectin-8 Antibody (C-8): sc-377133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
galectin-8 Double Nickase Plasmid (h) | sc-401785-NIC | 20 µg | $410.00 | |||
galectin-8 Double Nickase Plasmid (h2) | sc-401785-NIC-2 | 20 µg | $410.00 |
LGALS8 encodes galectin-8, a tandem-repeat β-galactoside–binding lectin that functions as a glycan-dependent sensor of membrane damage and a modulator of cell–cell and cell–matrix interactions. Galectin-8 participates in selective autophagy by recognizing exposed glycans on ruptured endomembranes and recruiting downstream effectors that promote xenophagy and clearance of damaged vesicles. It also influences integrin signaling, cell adhesion, and trafficking, linking extracellular glycoprotein recognition to intracellular stress-response pathways. Dysregulated LGALS8 expression and lectin-mediated signaling have been associated with inflammatory processes, pathogen–host interactions, and cancer-related phenotypes such as altered adhesion and invasion, supporting mechanistic studies in immune and epithelial contexts.
galectin-8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LGALS8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LGALS8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LGALS8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LGALS8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.