Eps15 Antibody (17): sc-135898
- Eps15 Antibody (17) is a mouse monoclonal IgG2a provided at 50 µg/0.5 ml
- raised against amino acids 694-888 of Eps15 of human origin
- recommended for detection of Eps15 of mouse, rat and human origin by WB, IP and IF; not recommended for IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Eps15 Antibody (17). This work is in progress.
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Eps15 Antibody (17) is a mouse monoclonal IgG2a antibody that recognizes Eps15 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Eps15 plays a crucial role in regulating cellular processes, particularly in receptor tyrosine kinase (RTK) signaling pathways, which are essential for growth regulation and cellular response to external stimuli. Misregulation of these pathways can lead to cellular transformation and cancer, making anti-Eps15 antibody (17) significant in cancer research. Eps15 becomes tyrosine phosphorylated following EGF stimulation, indicating active involvement in signaling cascades. Eps15 interacts with Crk, a key adaptor protein, through a conserved proline-rich domain, which is characteristic of Crk binding proteins. This interaction suggests Eps15 may influence EGF effects on cell proliferation, as overexpression of Eps15 in NIH/3T3 cells induces cellular transformation. Eps15′s structural features, including its proline-rich domain, may facilitate interactions with other signaling molecules, although the functional significance of its PH and SH3 domains remains to be fully elucidated. Anti-Eps15 antibody (17) serves as an invaluable tool for researchers exploring RTK signaling mechanisms and their implications in cellular growth and cancer biology.
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Eps15 Antibody (17) References:
- Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development. | Koh, TW., et al. 2007. J Cell Biol. 178: 309-22. PMID: 17620409
- The endocytic adaptor Eps15 controls marginal zone B cell numbers. | Pozzi, B., et al. 2012. PLoS One. 7: e50818. PMID: 23226392
- p38-Mediated phosphorylation of Eps15 endocytic adaptor protein. | Zhou, Y., et al. 2014. FEBS Lett. 588: 131-7. PMID: 24269888
- Over-expression of EPS15 is a favorable prognostic factor in breast cancer. | Dai, X., et al. 2015. Mol Biosyst. 11: 2978-85. PMID: 26289382
- Redundant and nonredundant organismal functions of EPS15 and EPS15L1. | Milesi, C., et al. 2019. Life Sci Alliance. 2: PMID: 30692166
- Cellular functions and intrinsic attributes of the ATP-binding Eps15 homology domain-containing proteins. | Bhattacharyya, S. and Pucadyil, TJ. 2020. Protein Sci. 29: 1321-1330. PMID: 32223019
- Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway. | Enshoji, M., et al. 2022. J Cell Biol. 221: PMID: 35984332
- lncRNA EPS15-AS1 affects the biological behavior of liver cancer stem cells by regulating EPS15 expression. | Liu, G., et al. 2022. Am J Transl Res. 14: 7324-7335. PMID: 36398248
- USP9X Controls EGFR Fate by Deubiquitinating the Endocytic Adaptor Eps15. | Savio, MG., et al. 2023. Curr Biol. 33: 5533. PMID: 38113830
- EPS15-AS1 Inhibits AKR1B1 Expression to Enhance Ferroptosis in Hepatocellular Carcinoma Cells. | Man, Q., et al. 2024. J Cancer. 15: 1030-1040. PMID: 38230218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eps15 Antibody (17) | sc-135898 | 50 µg/0.5 ml | $322.00 |