EMILIN-2 CRISPR/Cas9 KO Plasmid (h): sc-406521
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMILIN-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EMILIN-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMILIN-2 Antibody (3D9): sc-517180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMILIN-2 CRISPR/Cas9 KO Plasmid (h) | sc-406521 | 20 µg | $397.00 |
Overview
EMILIN2 encodes EMILIN-2, an extracellular matrix glycoprotein enriched in elastic fiber–associated microfibrils that contributes to matrix organization and cell–matrix signaling. EMILIN-2 interfaces with integrin-mediated adhesion and mechanotransduction, influencing cytoskeletal dynamics, migration, and tissue remodeling. Through these extracellular cues, EMILIN2 can modulate pathways linked to inflammation and vascular and stromal homeostasis, including processes that shape angiogenic and fibrotic responses. Dysregulated EMILIN2 expression and altered matrix architecture have been associated with tumor microenvironment remodeling and cardiovascular-related extracellular matrix defects, supporting its relevance in disease mechanism research.
EMILIN-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EMILIN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EMILIN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EMILIN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EMILIN-2 protein expression.
This CRISPR knockout system enables efficient generation of EMILIN2-deficient cell models for investigation of EMILIN-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EMILIN2 exon(s) critical for EMILIN-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EMILIN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EMILIN-2 CRISPR/Cas9 KO Plasmid (h) and EMILIN-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EMILIN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EMILIN-2 HDR Plasmid (h) and EMILIN-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EMILIN2 homology arms to support homology-directed repair at defined EMILIN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.