Elf-1 Antibody (D-4): sc-373772

Elf-1 Antibody (D-4) is a mouse monoclonal IgG2a light chain antibody that detects Elf-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Elf-1 is a crucial member of the Ets family of transcription factors, which play significant roles in regulating gene expression during various cellular processes, particularly in the immune system. Elf-1 is primarily located in the nucleus, where anti-Elf-1 antibody (D-4) can detect specific DNA sequence binding that modulates the transcription of target genes, including those involved in T cell activation. This nuclear localization is vital as Elf-1 interacts with other transcriptional regulators and chromatin remodeling complexes, thereby influencing the expression of genes necessary for immune responses. Additionally, Elf-1′s ability to bind to the retinoblastoma protein (Rb) highlights a role in cell cycle regulation and tumor suppression, making Elf-1 an important factor in both normal cellular function and oncogenesis. With diverse applications and critical biological functions, Elf-1 (D-4) antibody serves as an essential tool for researchers studying gene regulation and immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Elf-1 Antibody (D-4) References:

1. Phosphorylation and O-linked glycosylation of Elf-1 leads to its translocation to the nucleus and binding to the promoter of the TCR zeta-chain.  |  Juang, YT., et al. 2002. J Immunol. 168: 2865-71. PMID: 11884456
2. Critical role for the Ets transcription factor ELF-1 in the development of tumor angiogenesis.  |  Huang, X., et al. 2006. Blood. 107: 3153-60. PMID: 16352813
3. Expression of Elf-1 and survivin in non-small cell lung cancer and their relationship to intratumoral microvessel density.  |  Yang, DX., et al. 2010. Chin J Cancer. 29: 396-402. PMID: 20346215
4. PTH regulates myleoid ELF-1-like factor (MEF)-induced MAB-21-like-1 (MAB21L1) expression through the JNK1 pathway.  |  Kim, BG., et al. 2011. J Cell Biochem. 112: 2051-61. PMID: 21465527
5. The ETS Factor Myeloid Elf-1-Like Factor (MEF)/Elf4 Is Transcriptionally and Functionally Activated by Hypoxia.  |  Suico, MA., et al. 2016. Biol Pharm Bull. 39: 641-7. PMID: 27040637
6. Molecular regulation of the human IL-3 gene: inducible T cell-restricted expression requires intact AP-1 and Elf-1 nuclear protein binding sites.  |  Gottschalk, LR., et al. 1993. J Exp Med. 178: 1681-92. PMID: 8228815
7. Importance of low affinity Elf-1 sites in the regulation of lymphoid-specific inducible gene expression.  |  John, S., et al. 1996. J Exp Med. 183: 743-50. PMID: 8642278
8. Regulation of topographic projection in the brain: Elf-1 in the hippocamposeptal system.  |  Gao, PP., et al. 1996. Proc Natl Acad Sci U S A. 93: 11161-6. PMID: 8855326
9. Elf-1 regulates basal expression from the T cell antigen receptor zeta-chain gene promoter.  |  Rellahan, BL., et al. 1998. J Immunol. 160: 2794-801. PMID: 9510181
10. Transcriptional regulation of the stem cell leukemia gene by PU.1 and Elf-1.  |  Bockamp, EO., et al. 1998. J Biol Chem. 273: 29032-42. PMID: 9786909

The preferred Elf-1 antibody is Elf-1 Antibody (C-4): sc-133096.