Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Eg5 Double Nickase Plasmid (h): sc-402276-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Eg5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Eg5 Double Nickase Plasmid (h) and Eg5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting . One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Eg5 Antibody (F-1): sc-374212
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eg5 Double Nickase Plasmid (h) | sc-402276-NIC | 20 µg | $410.00 | |||
Eg5 Double Nickase Plasmid (h2) | sc-402276-NIC-2 | 20 µg | $410.00 |
Eg5, encoded by the human KIF11 gene, is a mitotic kinesin motor essential for bipolar spindle assembly and centrosome separation during cell division. By crosslinking and sliding antiparallel microtubules, Eg5 drives spindle pole separation and supports accurate chromosome congression and segregation. Its activity integrates with spindle assembly checkpoint control and microtubule dynamics to maintain genome stability. Dysregulated Eg5 function or expression is associated with aberrant mitosis, aneuploidy, and proliferative phenotypes relevant to cancer biology and chromosomal instability research.
Eg5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within . When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of -disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.