Dlx-1 CRISPR/Cas9 KO Plasmid (m): sc-420012
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dlx-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dlx-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dlx-1 Antibody (F-16): sc-81959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dlx-1 CRISPR/Cas9 KO Plasmid (m) | sc-420012 | 20 µg | $397.00 |
Overview
Dlx1 encodes the homeobox transcription factor Dlx-1, a DNA-binding regulator that coordinates gene expression programs required for embryonic patterning and neurodevelopment. In mouse, Dlx-1 is prominently involved in forebrain development, including specification, migration, and differentiation of GABAergic interneuron lineages, and it contributes to craniofacial and branchial arch morphogenesis. Through transcriptional control of downstream developmental networks, Dlx-1 influences cell fate decisions, tissue patterning, and maturation of neuronal circuitry. Dysregulated DLX1-associated gene programs have been linked in the literature to altered neurodevelopmental trajectories and oncogenic phenotypes in certain cellular contexts, making it relevant for mechanistic studies of differentiation and disease-associated transcriptional states.
Dlx-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dlx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dlx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dlx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dlx-1 protein expression.
This CRISPR knockout system enables efficient generation of Dlx1-deficient cell models for investigation of Dlx-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dlx1 exon(s) critical for Dlx-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dlx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dlx-1 CRISPR/Cas9 KO Plasmid (m) and Dlx-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dlx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dlx-1 HDR Plasmid (m) and Dlx-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dlx1 homology arms to support homology-directed repair at defined Dlx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.