DET1 CRISPR/Cas9 KO Plasmid (h): sc-406735
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DET1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DET1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DET1 Antibody (F-2): sc-514348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DET1 CRISPR/Cas9 KO Plasmid (h) | sc-406735 | 20 µg | $397.00 |
Overview
DET1 (de-etiolated homolog 1) encodes a conserved nuclear regulator that functions as a scaffold within the CUL4–DDB1 E3 ubiquitin ligase machinery, helping coordinate ubiquitin-dependent protein turnover. Through this complex, DET1 contributes to transcriptional control and chromatin-associated processes that influence cell cycle progression, DNA damage responses, and proteostasis. DET1 activity intersects with signaling programs regulated by ubiquitination, including pathways impacting proliferative and stress-adaptive gene expression. Dysregulation of DET1-linked ubiquitin ligase function has been connected to altered transcriptional networks and genomic stability phenotypes relevant to cancer biology and other disorders characterized by proteostasis imbalance.
DET1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DET1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DET1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DET1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DET1 protein expression.
This CRISPR knockout system enables efficient generation of DET1-deficient cell models for investigation of DET1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DET1 exon(s) critical for DET1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DET1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DET1 CRISPR/Cas9 KO Plasmid (h) and DET1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DET1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DET1 HDR Plasmid (h) and DET1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DET1 homology arms to support homology-directed repair at defined DET1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.