Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDIT3/GADD153/CHOP CRISPR/Cas9 KO Plasmid (m): sc-419970
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDIT3/GADD153/CHOP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDIT3/GADD153/CHOP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDIT3/GADD153/CHOP Antibody (B-3): sc-7351
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDIT3/GADD153/CHOP CRISPR/Cas9 KO Plasmid (m) | sc-419970 | 20 µg | $397.00 |
Overview
Mouse Ddit3 encodes DDIT3 (also known as GADD153/CHOP), a stress-inducible bZIP transcription factor that heterodimerizes with C/EBP family members to reprogram gene expression during cellular stress. It is a central effector of the unfolded protein response downstream of PERK–eIF2α–ATF4 signaling and integrates endoplasmic reticulum stress with oxidative stress, nutrient deprivation, and DNA damage pathways. DDIT3 regulates apoptosis, autophagy, and cell-cycle control, influencing mitochondrial integrity and transcriptional programs that determine cell fate. Altered DDIT3 activity has been implicated in inflammatory and metabolic phenotypes and is widely used as a mechanistic readout in models of neurodegeneration, diabetes-related β-cell stress, and tumor microenvironment stress adaptation.
DDIT3/GADD153/CHOP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ddit3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ddit3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ddit3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDIT3/GADD153/CHOP protein expression.
This CRISPR knockout system enables efficient generation of Ddit3-deficient cell models for investigation of DDIT3/GADD153/CHOP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ddit3 exon(s) critical for DDIT3/GADD153/CHOP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ddit3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDIT3/GADD153/CHOP CRISPR/Cas9 KO Plasmid (m) and DDIT3/GADD153/CHOP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ddit3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDIT3/GADD153/CHOP HDR Plasmid (m) and DDIT3/GADD153/CHOP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ddit3 homology arms to support homology-directed repair at defined Ddit3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.