DABP Antibody (H-6): sc-390146
- DABP Antibody (H-6) is a mouse monoclonal IgG2a κ DABP antibody provided at 200 µg/ml
- raised against amino acids 35-74 mapping near the N-terminus of DABP of human origin
- DABP Antibody (H-6) is recommended for detection of DABP of human origin by WB, IP, IF and ELISA
- Anti-DABP Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390146 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DABP Antibody (H-6) for WB applications. These reagents are now offered in bundles with DABP Antibody (H-6) (see ordering information below).
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SEE ALSO...
DABP Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects DABP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DABP (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DABP, also known as D-boxBP, D site-binding protein, and Albumin D-element-binding protein, is a 325 amino acid member of the bZIP family (PAR subfamily) that contains a single bZIP domain. DABP functions as a transcriptional activator, specifically recognizing and binding to the promoter sequence 5′-RTTAYGTAAY-3′ in the promoter regions of key genes such as albumin, CYP2A4, and CYP2A5. While DABP is not essential for generating circadian rhythms, DABP significantly influences the modulation of important clock output genes, potentially serving as a direct target for regulation by the circadian pacemaker component Clock. Notably, mice lacking bZip PAR gene products, including DABP, EPAS-1, and TEF, exhibit heightened susceptibility to generalized spontaneous and audiogenic epilepsies, likely due to the role of bZip PAR in targeting the gene encoding pyridoxal kinase. This enzyme is vital for converting vitamin B6 derivatives into pyridoxal phosphate, a crucial coenzyme involved in amino acid and neurotransmitter metabolism, underscoring DABP′s importance in both transcriptional regulation and metabolic processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DABP Antibody (H-6) References:
- The ins and outs of circadian timekeeping. | Brown, SA. and Schibler, U. 1999. Curr Opin Genet Dev. 9: 588-94. PMID: 10508692
- Liver-enriched transcription factors in liver function and development. Part II: the C/EBPs and D site-binding protein in cell cycle control, carcinogenesis, circadian gene regulation, liver regeneration, apoptosis, and liver-specific gene regulation. | Schrem, H., et al. 2004. Pharmacol Rev. 56: 291-330. PMID: 15169930
- The loss of circadian PAR bZip transcription factors results in epilepsy. | Gachon, F., et al. 2004. Genes Dev. 18: 1397-412. PMID: 15175240
- Chromosomal localization in man and rat of the genes encoding the liver-enriched transcription factors C/EBP, DBP, and HNF1/LFB-1 (CEBP, DBP, and transcription factor 1, TCF1, respectively) and of the hepatocyte growth factor/scatter factor gene (HGF). | Szpirer, C., et al. 1992. Genomics. 13: 293-300. PMID: 1535333
- Clock gene expression in the submandibular glands. | Furukawa, M., et al. 2005. J Dent Res. 84: 1193-7. PMID: 16304453
- The circadian PAR-domain basic leucine zipper transcription factors DBP, TEF, and HLF modulate basal and inducible xenobiotic detoxification. | Gachon, F., et al. 2006. Cell Metab. 4: 25-36. PMID: 16814730
- Chromosomal localization and cDNA cloning of the human DBP and TEF genes. | Khatib, ZA., et al. 1994. Genomics. 23: 344-51. PMID: 7835883
- Genomic structure of the human D-site binding protein (DBP) gene. | Shutler, G., et al. 1996. Genomics. 34: 334-9. PMID: 8786133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DABP Antibody (H-6) | sc-390146 | 200 µg/ml | $322.00 | |||
DABP Antibody (H-6): m-IgG Fc BP-HRP Bundle | sc-530079 | 200 µg Ab; 10 µg BP | $361.00 | |||
DABP Antibody (H-6): m-IgGκ BP-HRP Bundle | sc-523425 | 200 µg Ab, 40 µg BP | $361.00 | |||
DABP Antibody (H-6): m-IgG2a BP-HRP Bundle | sc-547427 | 200 µg Ab; 10 µg BP | $361.00 | |||
DABP Antibody (H-6) X | sc-390146 X | 200 µg/0.1 ml | $322.00 | |||
DABP Antibody (H-6) AC | sc-390146 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DABP Antibody (H-6) HRP | sc-390146 HRP | 200 µg/ml | $322.00 | |||
DABP Antibody (H-6) FITC | sc-390146 FITC | 200 µg/ml | $336.00 | |||
DABP Antibody (H-6) PE | sc-390146 PE | 200 µg/ml | $349.00 | |||
DABP Antibody (H-6) Alexa Fluor® 488 | sc-390146 AF488 | 200 µg/ml | $364.00 | |||
DABP Antibody (H-6) Alexa Fluor® 546 | sc-390146 AF546 | 200 µg/ml | $364.00 | |||
DABP Antibody (H-6) Alexa Fluor® 594 | sc-390146 AF594 | 200 µg/ml | $364.00 | |||
DABP Antibody (H-6) Alexa Fluor® 647 | sc-390146 AF647 | 200 µg/ml | $364.00 | |||
DABP Antibody (H-6) Alexa Fluor® 680 | sc-390146 AF680 | 200 µg/ml | $364.00 | |||
DABP Antibody (H-6) Alexa Fluor® 790 | sc-390146 AF790 | 200 µg/ml | $364.00 |