Cochlin CRISPR/Cas9 KO Plasmid (h): sc-405944
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cochlin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cochlin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cochlin CRISPR/Cas9 KO Plasmid (h) | sc-405944 | 20 µg | $397.00 |
Overview
COCH encodes cochlin, a secreted extracellular matrix protein highly enriched in the inner ear where it contributes to structural organization and mechanical homeostasis of auditory and vestibular tissues. Cochlin contains LCCL and von Willebrand factor A–like domains that support matrix interactions and may influence cell–matrix signaling, tissue remodeling, and inflammatory responses. Altered COCH function is linked to hereditary hearing and balance disorders, and cochlin deposition has been associated with degenerative changes in the cochlea and vestibular apparatus. As a matrix-associated protein, cochlin is studied in pathways governing extracellular matrix assembly, mechanotransduction, and stress responses in sensory epithelia.
Cochlin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COCH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COCH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COCH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cochlin protein expression.
This CRISPR knockout system enables efficient generation of COCH-deficient cell models for investigation of Cochlin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COCH exon(s) critical for Cochlin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COCH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cochlin CRISPR/Cas9 KO Plasmid (h) and Cochlin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COCH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cochlin HDR Plasmid (h) and Cochlin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COCH homology arms to support homology-directed repair at defined COCH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.