Yes Antibody (F-7): sc-8403

c-Yes Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects c-Yes protein of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. c-Yes (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. c-Yes protein plays a crucial role in cellular signaling pathways, particularly in the regulation of cell proliferation, differentiation, and survival. As a member of the Src family of kinases, c-Yes is involved in transducing signals that influence various cellular processes, including motility and adhesion, which are vital for tissue development and repair. c-Yes gene is located on chromosome 18p11.31-p11.21 and encodes a 543 amino acid protein that shares over 80% homology with c-Src, another well-known proto-oncogenic kinase. Structural similarities between these kinases, particularly in their SH3 and SH2 domains, allow for specific substrate interactions, which are essential for their function in signaling pathways. Understanding c-Yes role in these processes is important for elucidating potential implications in cancer biology and therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Yes Antibody (F-7) References:

1. Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells.  |  Chen, YH., et al. 2002. Mol Biol Cell. 13: 1227-37. PMID: 11950934
2. Regulation of the human sperm tyrosine kinase c-yes. Activation by cyclic adenosine 3',5'-monophosphate and inhibition by Ca(2+).  |  Leclerc, P. and Goupil, S. 2002. Biol Reprod. 67: 301-7. PMID: 12080032
3. c-yes protein kinase is associated with a 38 kD protein in cerebellum.  |  Grandori, C., et al. 1991. Oncogene. 6: 1063-6. PMID: 2067844
4. Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells.  |  Sancier, F., et al. 2011. PLoS One. 6: e17237. PMID: 21390316
5. Bioactive Phytoconstituents as Potent Inhibitors of Tyrosine-Protein Kinase Yes (YES1): Implications in Anticancer Therapeutics.  |  Yang, C., et al. 2022. Molecules. 27: PMID: 35630545
6. Tyrosine-protein kinase Yes controls endothelial junctional plasticity and barrier integrity by regulating VE-cadherin phosphorylation and endocytosis.  |  Jin, Y., et al. 2022. Nat Cardiovasc Res. 1: 1156-1173. PMID: 37936984
7. Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells.  |  Wu, T., et al. 2024. World J Stem Cells. 16: 267-286. PMID: 38577236
8. Elevated expression of protein tyrosine kinase c-Yes, but not c-Src, in human malignant melanoma.  |  Loganzo, F., et al. 1993. Oncogene. 8: 2637-44. PMID: 7690926
9. Inactivation of c-Yes tyrosine kinase by elevation of intracellular calcium levels.  |  Zhao, Y., et al. 1993. Mol Cell Biol. 13: 7507-14. PMID: 8246968
10. Stimulation of the protein tyrosine kinase c-Yes but not c-Src by neurotrophins in human brain-metastatic melanoma cells.  |  Marchetti, D., et al. 1998. Oncogene. 16: 3253-60. PMID: 9681823

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Yes Antibody (F-7) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-Yes Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Yes Antibody (F-7) Specifications

Alternate Product Name: Tyrosine Protein Kinase Yes

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-18

Epitope Target: c-Yes

Epitope Species: human

Product Reactivity/Detection: c-Yes p62

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for Yes Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine

The preferred Yes antibody is Yes Antibody (C-10): sc-46674.