Arnt 1 Antibody (G-3): sc-17812

Arnt 1 Antibody (G-3) is a mouse monoclonal IgG2a antibody that detects Arnt 1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Arnt 1, also known as aryl hydrocarbon receptor nuclear translocator, plays a crucial role in cellular responses to environmental toxins and is essential for the function of the aryl hydrocarbon receptor (AhR) pathway. This pathway regulates the expression of genes involved in xenobiotic metabolism, including CYP1A1, which is responsible for the detoxification of harmful compounds. Arnt 1′s structure includes a basic helix-loop-helix domain, which facilitates dimerization with AhR, and two PAS domains that are critical for interaction with other proteins and DNA. Nuclear translocation of Arnt 1 upon ligand binding enables activation of target genes that help protect cells from damage caused by environmental pollutants. Anti-Arnt 1 antibody (G-3) serves as an invaluable tool for researchers studying gene regulation mechanisms and cellular response to xenobiotics, providing insights into both normal physiological processes and potential impacts of environmental toxins on human health.

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Arnt 1 Antibody (G-3) References:

1. Molecular characterization and tissue distribution of aryl hydrocarbon receptor nuclear translocator isoforms, ARNT1 and ARNT2, and identification of novel splice variants in common cormorant (Phalacrocorax carbo).  |  Lee, JS., et al. 2007. Comp Biochem Physiol C Toxicol Pharmacol. 145: 379-93. PMID: 17337252
2. Carbohydrate-responsive element-binding protein (ChREBP) is a negative regulator of ARNT/HIF-1beta gene expression in pancreatic islet beta-cells.  |  Noordeen, NA., et al. 2010. Diabetes. 59: 153-60. PMID: 19833882
3. Evolutionary conserved regulation of HIF-1β by NF-κB.  |  van Uden, P., et al. 2011. PLoS Genet. 7: e1001285. PMID: 21298084
4. Hypoxia-inducible factor-1β (HIF-1β) is upregulated in a HIF-1α-dependent manner in 518A2 human melanoma cells under hypoxic conditions.  |  Mandl, M., et al. 2013. Biochem Biophys Res Commun. 434: 166-72. PMID: 23541582
5. The action of HIF-3α variants on HIF-2α-HIF-1β heterodimer formation is directly probed in live cells.  |  Kim, SH., et al. 2015. Exp Cell Res. 336: 329-37. PMID: 26160453
6. Molecular cloning and characterization of two ARNT (ARNT-1 and ARNT-2) genes in Atlantic croaker and their expression during coexposure to hypoxia and PCB77.  |  Rahman, MS. and Thomas, P. 2019. Environ Toxicol. 34: 160-171. PMID: 30334616
7. Hypoxic Gene Signature of Primary and Metastatic Melanoma Cell Lines: Focusing on HIF-1β and NDRG-1.  |  Ercin, ME., et al. 2019. Balkan Med J. 37: 15-23. PMID: 31594284
8. HIF-1β Positively Regulates NF-κB Activity via Direct Control of TRAF6.  |  D'Ignazio, L., et al. 2020. Int J Mol Sci. 21: PMID: 32344511
9. Effects of Myeloid Hif-1β Deletion on the Intestinal Microbiota in Mice under Environmental Hypoxia.  |  Han, N., et al. 2020. Infect Immun. 89: PMID: 33106294
10. DDX5 enhances HIF-1 activity by promoting the interaction of HIF-1α with HIF-1β and recruiting the resulting heterodimer to its target gene loci.  |  Shirai, Y., et al. 2024. Biol Cell. 116: e2300077. PMID: 38031929

The preferred Arnt 1 antibody is Arnt 1 Antibody (A-3): sc-17811.