ARG1/Arginase 1 CRISPR/Cas9 KO Plasmid (bovine): sc-437305

Overview

ARG1/Arginase 1 encodes a manganese-dependent ureohydrolase that catalyzes conversion of L-arginine to urea and ornithine, functioning as a key terminal enzyme of the urea cycle and a regulator of nitrogen disposal. By controlling arginine bioavailability, ARG1 influences polyamine and proline biosynthesis via ornithine, links amino acid metabolism to collagen production, and modulates nitric oxide signaling through competition with nitric oxide synthases. In bovine systems, ARG1 activity is relevant to hepatic metabolic homeostasis and to immunometabolic programs in myeloid cells, where arginine catabolism can shape inflammatory tone and tissue remodeling. Dysregulated arginase-driven arginine depletion has been associated with impaired immune effector functions and altered metabolic stress responses, supporting mechanistic studies in liver and immune models.

ARG1/Arginase 1 CRISPR/Cas9 KO Plasmid (bovine) is a pool of plasmids designed for targeted disruption of the gene in bovine cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARG1/Arginase 1 protein expression.

This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of ARG1/Arginase 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting exon(s) critical for ARG1/Arginase 1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by ARG1/Arginase 1 CRISPR/Cas9 KO Plasmid (bovine) and ARG1/Arginase 1 CRISPR/Cas9 KO Plasmid (bovine2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by ARG1/Arginase 1 HDR Plasmid (bovine) and ARG1/Arginase 1 HDR Plasmid (bovine2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.