Amphiphysin II Antibody (99D): sc-13575

Amphiphysin II Antibody (99D) is a mouse monoclonal IgG2b kappa light chain antibody that detects Amphiphysin II across mouse, rat, human, and avian species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Amphiphysin II antibody (99D) is available in a non-conjugated form for versatile experimental use. Amphiphysin II is a brain-enriched protein playing a significant role in synaptic vesicle recycling, essential for neurotransmission. Amphiphysin II (99D) monoclonal antibody recognizes a protein that functions as a dimer and features a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. The interaction between Amphiphysin II and the GTPase Dynamin, mediated through the SH3 domain, enables synaptic vesicle recycling in neurons by facilitating Dynamin binding to Clathrin-coated pits, leading to vesicle budding. A splice variant of Amphiphysin II, lacking Clathrin or adaptor interactions, predominantly expresses in muscle tissue and plays a crucial role in T tubule network formation and stabilization, demonstrating diverse functional roles in cellular dynamics.

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Amphiphysin II Antibody (99D) References:

1. Bridging integrator 1 (BIN1): form, function, and Alzheimer's disease.  |  Tan, MS., et al. 2013. Trends Mol Med. 19: 594-603. PMID: 23871436
2. Amphiphysin 2 (BIN1) in physiology and diseases.  |  Prokic, I., et al. 2014. J Mol Med (Berl). 92: 453-63. PMID: 24590001
3. The Mechanistic Role of Bridging Integrator 1 (BIN1) in Alzheimer's Disease.  |  Gao, P., et al. 2021. Cell Mol Neurobiol. 41: 1431-1440. PMID: 32719966
4. Lack of association between bridging integrator 1 (BIN1) rs744373 polymorphism and tau-PET load in cognitively intact older adults.  |  Schaeverbeke, J., et al. 2022. Alzheimers Dement (N Y). 8: e12227. PMID: 35229019
5. BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.  |  Sudwarts, A., et al. 2022. Mol Neurodegener. 17: 33. PMID: 35526014
6. Bridging Integrator 1 (BIN1, rs6733839) and Sex Are Moderators of Vascular Health Predictions of Memory Aging Trajectories.  |  Heal, M., et al. 2022. J Alzheimers Dis. 89: 265-281. PMID: 35871342
7. Bridging integrator 1 fragment accelerates tau aggregation and propagation by enhancing clathrin-mediated endocytosis in mice.  |  Zhang, X., et al. 2024. PLoS Biol. 22: e3002470. PMID: 38206965
8. Amphiphysin-2 (BIN1) functions and defects in cardiac and skeletal muscle.  |  Giraud, Q. and Laporte, J. 2024. Trends Mol Med. 30: 579-591. PMID: 38514365

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Amphiphysin II Antibody (99D) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Amphiphysin II Antibody (99D) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Amphiphysin II Antibody (99D) Specifications

Alternate Product Name: Amphiphysin II

Product Clone Name: 99D

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Amphiphysin II

Epitope Species: human

Product Reactivity/Detection: Amphiphysin II splice variants 1-10

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Amphiphysin II Antibody (99D): N/A

The preferred Amphiphysin II antibody is Amphiphysin II Antibody (2F11): sc-23918.