Amphiphysin I Antibody (D-6): sc-390282

Amphiphysin I Antibody (D-6) is a mouse monoclonal IgG1 antibody that detects Amphiphysin I in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Amphiphysin I is a crucial brain-enriched protein that plays a significant role in synaptic vesicle recycling, a process vital for neurotransmission and communication between neurons. Amphiphysin I functions as a dimer and features a unique structure that includes a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. Amphiphysin I interaction with the GTPase Dynamin via its SH3 domain is essential for proper binding of Dynamin to Clathrin-coated pits, facilitating the budding of synaptic vesicles. Beyond brain function, Amphiphysin I is implicated in membrane bending and curvature stabilization in various tissues, highlighting its importance in cellular dynamics. Notably, mammalian Amphiphysins, including Amphiphysin I and its isoform Amphiphysin II, share a similar overall structure, yet exhibit distinct functional roles, particularly in muscle tissue where a splice variant of Amphiphysin II contributes to the formation and stabilization of the T tubule network.

The preferred Amphiphysin I antibody is Amphiphysin I Antibody (G-4): sc-376402.