alpha Tubulin Antibody (3H3085): sc-69970

α Tubulin Antibody (3H3085) is a rat monoclonal IgG2a antibody that specifically targets α Tubulin, a key protein in the structure of microtubules, from mouse, rat, human, and yeast sources. Anti-α Tubulin antibody (3H3085) is raised against full-length, purified α Tubulin derived from Saccharomyces cerevisiae and is effective for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC), with a particular recommendation for detecting the tyrosinated form of α Tubulin. As a major cytoskeletal component, α Tubulin partners with β Tubulin to form heterodimers that assemble into microtubules, which are essential for various cellular functions such as intracellular transport, cell division, and maintaining cell shape. Post-translational modifications of α Tubulin, particularly tyrosination and detyrosination, are crucial for regulating microtubule stability and interactions with motor proteins like kinesin and dynein. These modifications help control the dynamic behavior of microtubules, which is critical for processes such as cell division, mitotic spindle formation, and neuronal signaling. Additionally, α Tubulin′s role in the centrosome and cilia underscores its importance in microtubule nucleation and organization, with specific isoforms localizing to distinct regions in the cell during the cell cycle. Known alternately as Tubulin alpha chain or TUBA1A, α Tubulin is also involved in organizing the structure of cilia and flagella, essential for cellular movement and signaling. α Tubulin monoclonal antibody (3H3085) serves as a valuable tool for studying the dynamics and regulation of microtubules, helping to unravel the molecular mechanisms underpinning cellular function and development.

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alpha Tubulin Antibody (3H3085) References:

1. Identification of alpha-tubulin as an hsp105alpha-binding protein by the yeast two-hybrid system.  |  Saito, Y., et al. 2003. Exp Cell Res. 286: 233-40. PMID: 12749852
2. Structure of the α-tubulin acetyltransferase, αTAT1, and implications for tubulin-specific acetylation.  |  Friedmann, DR., et al. 2012. Proc Natl Acad Sci U S A. 109: 19655-60. PMID: 23071314
3. The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells.  |  Mao, Y., et al. 2013. Chin J Cancer Res. 25: 770-6. PMID: 24385707
4. SIRT2 regulates microtubule stabilization in diabetic cardiomyopathy.  |  Yuan, Q., et al. 2015. Eur J Pharmacol. 764: 554-561. PMID: 26209361
5. Chorein interacts with α-tubulin and histone deacetylase 6, and overexpression preserves cell viability during nutrient deprivation in human embryonic kidney 293 cells.  |  Sasaki, N., et al. 2016. FASEB J. 30: 3726-3732. PMID: 27468757
6. Organization and structure of Volvox alpha-tubulin genes.  |  Mages, W., et al. 1988. Mol Gen Genet. 213: 449-58. PMID: 3185511
7. Perfluorooctane sulfonate induces autophagy-dependent lysosomal membrane permeabilization by weakened interaction between tyrosinated alpha-tubulin and spinster 1.  |  Dong, Z., et al. 2021. Food Chem Toxicol. 157: 112540. PMID: 34500008
8. Acetylated α-tubulin K394 regulates microtubule stability to shape the growth of axon terminals.  |  Saunders, HAJ., et al. 2022. Curr Biol. 32: 614-630.e5. PMID: 35081332
9. HDAC6 decreases H4K16 and α-tubulin acetylation during porcine oocyte maturation.  |  Zhang, S., et al. 2023. Cell Cycle. 22: 2057-2069. PMID: 37904550
10. Posttranslational processing of alpha-tubulin during axoplasmic transport in CNS axons.  |  Brown, BA., et al. 1982. J Cell Biol. 94: 159-64. PMID: 6181079

The preferred alpha Tubulin antibody is alpha Tubulin Antibody (TU-02): sc-8035.