Alix Antibody (3A9): sc-53538
- Alix Antibody (3A9) is a mouse monoclonal IgG1 κ, cited in 70 publications, provided at 200 µg/ml
- Alix Antibody (3A9) is recommended for detection of Alix of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Alix Antibody (3A9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Alix Antibody (3A9) for WB and IHC(P) applications. These reagents are now offered in bundles with Alix Antibody (3A9) (see ordering information below).
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Alix Antibody (3A9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Alix in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Alix (3A9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ALG-2-interacting protein, commonly known as Alix, plays a crucial role in cellular processes by facilitating the sorting and concentration of cargo proteins within multivesicular bodies, which are essential for the formation of vesicles that transport proteins to their appropriate destinations. This sorting mechanism is vital for maintaining cellular homeostasis and regulating apoptosis, as Alix interacts with key proteins involved in these processes, including ALG-2 and programmed cell death 6. Furthermore, Alix′s interactions with endosomal sorting complexes required for transport (ESCRT) proteins, such as Tsg101 and CHMP4, highlight Alix′s importance in the endocytic pathway and viral budding, particularly in the context of HIV-1. Anti-Alix antibody (3A9) enables researchers to gain insights into the dynamic roles of Alix in cellular trafficking and implications in various diseases, including cancer and viral infections.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Alix Antibody (3A9) References:
- Alix and ALG-2 make a link between endosomes and neuronal death. | Mahul-Mellier, AL., et al. 2009. Biochem Soc Trans. 37: 200-3. PMID: 19143631
- Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. | Baietti, MF., et al. 2012. Nat Cell Biol. 14: 677-85. PMID: 22660413
- ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes. | Larios, J., et al. 2020. J Cell Biol. 219: PMID: 32049272
- Alix is required for activity-dependent bulk endocytosis at brain synapses. | Laporte, MH., et al. 2022. PLoS Biol. 20: e3001659. PMID: 35658004
- Transforming Diversity, Equity, and Inclusion in Medical Education-Mayo Clinic Alix School of Medicine. | Valencia, EM. 2022. Mayo Clin Proc. 97: 1592-1594. PMID: 36058569
- Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation. | Elias, RD., et al. 2023. Sci Adv. 9: eadg3913. PMID: 37450591
- Vesicle-mediated transport of ALIX and ESCRT-III to the intercellular bridge during cytokinesis. | Pust, S., et al. 2023. Cell Mol Life Sci. 80: 235. PMID: 37523003
- SUMOylation-triggered ALIX activation modulates extracellular vesicles circTLCD4-RWDD3 to promote lymphatic metastasis of non-small cell lung cancer. | Diao, X., et al. 2023. Signal Transduct Target Ther. 8: 426. PMID: 37925421
- ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses. | Chen, X., et al. 2024. PLoS Pathog. 20: e1012103. PMID: 38489378
- Essential role of Alix in regulating cardiomyocyte exosome biogenesis under physiological and stress conditions. | Wang, X., et al. 2024. J Mol Cell Cardiol. 190: 35-47. PMID: 38593639
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Alix Antibody (3A9) | sc-53538 | 200 µg/ml | $322.00 | |||
Alix Antibody (3A9): m-IgG Fc BP-HRP Bundle | sc-526824 | 200 µg Ab; 10 µg BP | $361.00 | |||
Alix Antibody (3A9): m-IgG1 BP-HRP Bundle | sc-532197 | 200 µg Ab; 20 µg BP | $361.00 | |||
Alix Antibody (3A9) AC | sc-53538 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Alix Antibody (3A9) HRP | sc-53538 HRP | 200 µg/ml | $322.00 | |||
Alix Antibody (3A9) FITC | sc-53538 FITC | 200 µg/ml | $336.00 | |||
Alix Antibody (3A9) PE | sc-53538 PE | 200 µg/ml | $349.00 | |||
Alix Antibody (3A9) Alexa Fluor® 488 | sc-53538 AF488 | 200 µg/ml | $364.00 | |||
Alix Antibody (3A9) Alexa Fluor® 546 | sc-53538 AF546 | 200 µg/ml | $364.00 | |||
Alix Antibody (3A9) Alexa Fluor® 594 | sc-53538 AF594 | 200 µg/ml | $364.00 | |||
Alix Antibody (3A9) Alexa Fluor® 647 | sc-53538 AF647 | 200 µg/ml | $364.00 | |||
Alix Antibody (3A9) Alexa Fluor® 680 | sc-53538 AF680 | 200 µg/ml | $364.00 | |||
Alix Antibody (3A9) Alexa Fluor® 790 | sc-53538 AF790 | 200 µg/ml | $364.00 |