Aldehyde dehydrogenase 1-A1/ALDH1A1 CRISPR/Cas9 KO Plasmid (m): sc-419078

Overview

Aldh1a1 encodes aldehyde dehydrogenase 1-A1 (ALDH1A1), a cytosolic NAD(P)+-dependent enzyme that oxidizes a broad range of endogenous and xenobiotic aldehydes to their corresponding carboxylic acids, thereby supporting cellular detoxification and redox homeostasis. In mouse tissues, ALDH1A1 contributes to retinoid metabolism by catalyzing retinaldehyde to retinoic acid, linking the gene to transcriptional programs that influence differentiation, metabolism, and oxidative stress responses. ALDH1A1 activity intersects with lipid peroxidation and reactive aldehyde clearance pathways, helping limit carbonyl stress and downstream protein and DNA adduct formation. Altered ALDH1A1 expression or function has been associated with oxidative stress–related phenotypes and is frequently studied in contexts such as metabolic dysregulation, neurobiology, and tumor cell biology as a marker of cellular state.

Aldehyde dehydrogenase 1-A1/ALDH1A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aldh1a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aldh1a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aldh1a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldehyde dehydrogenase 1-A1/ALDH1A1 protein expression.

This CRISPR knockout system enables efficient generation of Aldh1a1-deficient cell models for investigation of Aldehyde dehydrogenase 1-A1/ALDH1A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Aldh1a1 exon(s) critical for Aldehyde dehydrogenase 1-A1/ALDH1A1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Aldh1a1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Aldehyde dehydrogenase 1-A1/ALDH1A1 CRISPR/Cas9 KO Plasmid (m) and Aldehyde dehydrogenase 1-A1/ALDH1A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aldh1a1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Aldehyde dehydrogenase 1-A1/ALDH1A1 HDR Plasmid (m) and Aldehyde dehydrogenase 1-A1/ALDH1A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aldh1a1 homology arms to support homology-directed repair at defined Aldh1a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.