Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AIP5 CRISPR/Cas9 KO Plasmid (h): sc-403772
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AIP5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AIP5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AIP5 Antibody (F-3): sc-390897
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AIP5 CRISPR/Cas9 KO Plasmid (h) | sc-403772 | 20 µg | $397.00 |
Overview
WWP1 encodes the HECT-type E3 ubiquitin ligase AIP5, a regulatory enzyme that catalyzes ubiquitin transfer to protein substrates to control their stability, localization, and signaling output. AIP5 participates in ubiquitin-dependent proteostasis and can influence receptor and kinase signaling cascades, transcriptional programs, and cellular stress responses through selective substrate turnover. By modulating protein quality control and signaling thresholds, WWP1 activity has been linked in the literature to cellular phenotypes relevant to proliferation, differentiation, and epithelial homeostasis. Dysregulation of ubiquitin ligase pathways involving WWP1 has been associated with disease-relevant mechanisms, including altered signal transduction and aberrant protein degradation networks studied in oncology and other complex disorders.
AIP5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WWP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WWP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WWP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AIP5 protein expression.
This CRISPR knockout system enables efficient generation of WWP1-deficient cell models for investigation of AIP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WWP1 exon(s) critical for AIP5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WWP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AIP5 CRISPR/Cas9 KO Plasmid (h) and AIP5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WWP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AIP5 HDR Plasmid (h) and AIP5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WWP1 homology arms to support homology-directed repair at defined WWP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.