Aiolos Antibody (G-5): sc-515945

Aiolos Antibody (G-5) is a mouse monoclonal IgG2a antibody that detects Aiolos in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Aiolos, also known as zinc finger protein Ikaros 3 or ZNFN1A3, is a crucial 509 amino acid nuclear protein that plays a significant role in lymphocyte differentiation, making Aiolos vital for proper immune function. Aiolos (G-5) antibody helps researchers study how Aiolos influences immune cell development, with mutations in Aiolos being linked to leukemogenesis, highlighting its relevance in cancer research. Predominantly expressed in the spleen, thymus, and peripheral blood leukocytes, Aiolos contains six C2H2-type zinc fingers, a structural motif essential for DNA binding and protein-protein interactions. These zinc fingers allow Aiolos to form homodimers and heterodimers with other Ikaros family members, such as Ikaros, Eos, and Pegasus, thereby coordinating complex transcriptional regulation in response to signaling events like T-cell activation. Anti-Aiolos antibody (G-5) serves as an invaluable tool for researchers studying immune cell differentiation and the molecular mechanisms underlying hematological malignancies.

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Aiolos Antibody (G-5) References:

1. Aiolos and Ikaros: regulators of lymphocyte development, homeostasis and lymphoproliferation.  |  Schmitt, C., et al. 2002. Apoptosis. 7: 277-84. PMID: 11997672
2. Ikaros, Aiolos and Helios: transcription regulators and lymphoid malignancies.  |  Rebollo, A. and Schmitt, C. 2003. Immunol Cell Biol. 81: 171-5. PMID: 12752680
3. Overexpression of AIOLOS inhibits cell proliferation and suppresses apoptosis in Nalm-6 cells.  |  Zhuang, Y., et al. 2014. Oncol Rep. 31: 1183-90. PMID: 24399134
4. Aiolos promotes anchorage independence by silencing p66Shc transcription in cancer cells.  |  Li, X., et al. 2014. Cancer Cell. 25: 575-89. PMID: 24823637
5. Upregulation of AIOLOS induces apoptosis and enhances etoposide chemosensitivity in Jurkat leukemia cells.  |  Zhuang, Y., et al. 2015. Oncol Rep. 33: 1319-25. PMID: 25524659
6. Aiolos collaborates with Blimp-1 to regulate the survival of multiple myeloma cells.  |  Hung, KH., et al. 2016. Cell Death Differ. 23: 1175-84. PMID: 26823144
7. Role of Aiolos and Ikaros in the Antitumor and Immunomodulatory Activity of IMiDs in Multiple Myeloma: Better to Lose Than to Find Them.  |  Cippitelli, M., et al. 2021. Int J Mol Sci. 22: PMID: 33499314
8. Interactome of Aiolos/Ikaros Reveals Combination Rationale of Cereblon Modulators with HDAC Inhibitors in DLBCL.  |  Hagner, PR., et al. 2022. Clin Cancer Res. 28: 3367-3377. PMID: 35583604
9. Disease-associated AIOLOS variants lead to immune deficiency/dysregulation by haploinsufficiency and redefine AIOLOS functional domains.  |  Kuehn, HS., et al. 2024. J Clin Invest. 134: PMID: 38015619
10. Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation.  |  Morgan, B., et al. 1997. EMBO J. 16: 2004-13. PMID: 9155026

The preferred Aiolos antibody is Aiolos Antibody (3H5-G7): sc-293421.