Agrin Antibody (D-2): sc-374117

Agrin Antibody (D-2) is a mouse monoclonal IgM antibody that detects Agrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Agrin antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Agrin plays a crucial role in the neuromuscular junction by facilitating the clustering of acetylcholine receptors (AChRs) on the muscle cell surface, which is essential for effective synaptic transmission and muscle contraction. This clustering is vital for the formation and maintenance of the neuromuscular junction, ensuring that signals from motor neurons are efficiently transmitted to muscle fibers. Agrin is primarily located in the basal lamina of muscle cells, where Agrin interacts with other proteins to orchestrate the assembly of the postsynaptic apparatus. Notably, Agrin exists in several splice variants, with specific forms exhibiting a high affinity for AChRs, which underscores the importance of post-translational modifications in regulating Agrin function. Additionally, the interaction between Agrin and the receptor tyrosine kinase MuSK is critical for the signaling pathways that lead to AChR aggregation, highlighting the intricate molecular mechanisms that govern neuromuscular junction formation and stability.

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Agrin Antibody (D-2) References:

1. The extracellular matrix protein agrin promotes heart regeneration in mice.  |  Bassat, E., et al. 2017. Nature. 547: 179-184. PMID: 28581497
2. Structural insights into the assembly of the agrin/LRP4/MuSK signaling complex.  |  Xie, T., et al. 2023. Proc Natl Acad Sci U S A. 120: e2300453120. PMID: 37252960
3. Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice.  |  Chen, J., et al. 2024. Cell Death Dis. 15: 201. PMID: 38461287
4. Agrin-Lrp4 pathway in hippocampal astrocytes restrains development of temporal lobe epilepsy through adenosine signaling.  |  Liu, ZY., et al. 2024. Cell Biosci. 14: 66. PMID: 38783336
5. Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation.  |  Ferns, M., et al. 1996. J Cell Biol. 132: 937-44. PMID: 8603924
6. Alternative splicing of agrin alters its binding to heparin, dystroglycan, and the putative agrin receptor.  |  Gesemann, M., et al. 1996. Neuron. 16: 755-67. PMID: 8607994
7. Agrin binding to alpha-dystroglycan. Domains of agrin necessary to induce acetylcholine receptor clustering are overlapping but not identical to the alpha-dystroglycan-binding region.  |  Hopf, C. and Hoch, W. 1996. J Biol Chem. 271: 5231-6. PMID: 8617807
8. Agrin signals at the junction.  |  Slater, CR. 1996. Nature. 381: 478-9. PMID: 8632818
9. Agrin acts via a MuSK receptor complex.  |  Glass, DJ., et al. 1996. Cell. 85: 513-23. PMID: 8653787
10. Defective neuromuscular synaptogenesis in agrin-deficient mutant mice.  |  Gautam, M., et al. 1996. Cell. 85: 525-35. PMID: 8653788
11. Neural agrin activates a high-affinity receptor in C2 muscle cells that is unresponsive to muscle agrin.  |  Bowen, DC., et al. 1996. J Neurosci. 16: 3791-7. PMID: 8656273
12. Alternative splicing of agrin regulates its binding to heparin alpha-dystroglycan, and the cell surface.  |  O'Toole, JJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 7369-74. PMID: 8693000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Agrin Antibody (D-2) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Agrin Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Agrin Antibody (D-2) Specifications

Alternate Product Name: AGRN

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1913-1942

Epitope Target: Agrin

Epitope Species: rat

Product Reactivity/Detection: Agrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Agrin Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin