ACLP/AEBP1 Double Nickase Plasmid (h): sc-403540-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACLP/AEBP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACLP/AEBP1 Double Nickase Plasmid (h) and ACLP/AEBP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AEBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACLP/AEBP1 Antibody (G-1): sc-271374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACLP/AEBP1 Double Nickase Plasmid (h) | sc-403540-NIC | 20 µg | $410.00 | |||
ACLP/AEBP1 Double Nickase Plasmid (h2) | sc-403540-NIC-2 | 20 µg | $410.00 |
AEBP1 encodes adipocyte enhancer-binding protein 1, also known as ACLP, a secreted and extracellular matrix–associated protein that binds collagen and contributes to matrix assembly, tissue remodeling, and cell–matrix adhesion. In stromal and mesenchymal contexts, AEBP1 is linked to pro-fibrotic and inflammatory signaling programs, influencing fibroblast activation, wound repair, and vascular extracellular matrix organization. Altered AEBP1/ACLP expression has been reported across fibrotic disorders and multiple tumor microenvironments, where it correlates with changes in stromal composition, invasiveness, and immune modulation. These features make AEBP1 a useful target for studying extracellular matrix biology, fibroblast–immune crosstalk, and remodeling pathways that shape tissue homeostasis and disease progression.
ACLP/AEBP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AEBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AEBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AEBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AEBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.